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Plasmid Files

pTriEx™-5

Insect, bacterial, and mammalian vector, encoding an N-terminal Strep-Tag® II-enterokinase cassette and a C-terminal 10xHis.

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pTriEx-5 Sequence and MappTriEx-5.dna
Map and Sequence File   
Sequence Author:  Novagen (EMD Millipore)
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 NcoI (2112) BstBI (2139) Acc65I (2167) KpnI (2171) BamHI (2173) PshAI (2183) HindIII (2192) EagI - NotI (2199) Eco53kI (2210) BseRI - SacI (2212) DraIII (2269) Bsu36I (2279) pTriEx™-5 5061 bp
NcoI  (2112)
1 site
C C A T G G G G T A C C
BstBI  (2139)
1 site
T T C G A A A A G C T T
Acc65I  (2167)
1 site
G G T A C C C C A T G G
KpnI  (2171)
1 site
G G T A C C C C A T G G
BamHI  (2173)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
PshAI  (2183)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
HindIII  (2192)
1 site
A A G C T T T T C G A A
EagI  (2199)
1 site
C G G C C G G C C G G C
NotI  (2199)
1 site
G C G G C C G C C G C C G G C G
Eco53kI  (2210)
1 site
G A G C T C C T C G A G
BseRI  (2212)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
SacI  (2212)
1 site
G A G C T C C T C G A G
DraIII  (2269)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
Bsu36I  (2279)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
AmpR
4139 .. 4999  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   4139 .. 4930  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4139 .. 4999  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   4931 .. 4999  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4139 .. 4999  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
baculovirus recombination region (lef2/ORF603)
181 .. 1008  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
181 .. 1008  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (ORF1629)
2624 .. 3329  =  706 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
2624 .. 3329  =  706 bp
contains part of ORF1629
ori
3539 .. 4126  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3539 .. 4126  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
Exon 2
1863 .. 2445  =  583 bp
Exon 2
1863 .. 2445  =  583 bp
CMV enhancer
1076 .. 1379  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
1076 .. 1379  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
1381 .. 1580  =  200 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
1381 .. 1580  =  200 bp
human cytomegalovirus (CMV) immediate early
promoter
intron
1699 .. 1862  =  164 bp
intron
1699 .. 1862  =  164 bp
Exon 1
1597 .. 1698  =  102 bp
Exon 1
1597 .. 1698  =  102 bp
T7 terminator
2564 .. 2611  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
T7 terminator
2564 .. 2611  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
ORF603
343 .. 948  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF603
343 .. 948  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF1629
2741 .. 3329  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF1629
2741 .. 3329  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
lef2
181 .. 305  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
lef2
181 .. 305  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
rabbit globin terminator
2443 .. 2554  =  112 bp
rabbit globin terminator
2443 .. 2554  =  112 bp
p10 promoter
1990 .. 2099  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
1990 .. 2099  =  110 bp
baculovirus promoter for expression in insect cells
MCS
2167 .. 2226  =  60 bp
multiple cloning site
MCS
2167 .. 2226  =  60 bp
multiple cloning site
10xHis
2237 .. 2266  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
10xHis
2237 .. 2266  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
lac operator
1950 .. 1974  =  25 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
1950 .. 1974  =  25 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
Strep-Tag II
2123 .. 2146  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an
engineered form of streptavidin
Strep-Tag II
2123 .. 2146  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an
engineered form of streptavidin
T7 promoter
1931 .. 1949  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1931 .. 1949  =  19 bp
promoter for bacteriophage T7 RNA polymerase
RBS
2101 .. 2106  =  6 bp
ribosome binding site
RBS
2101 .. 2106  =  6 bp
ribosome binding site
poly(A) signal
2421 .. 2426  =  6 bp
poly(A) signal
2421 .. 2426  =  6 bp
ATG
2114 .. 2116  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
2114 .. 2116  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
enterokinase site
2153 .. 2167  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
enterokinase site
2153 .. 2167  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
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