Plasmid Files

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NcoI  (2112) 1 site

BstBI  (2139) 1 site

Acc65I  (2167) 1 site

KpnI  (2171) 1 site

BamHI  (2173) 1 site

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.

PshAI  (2183) 1 site

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.

HindIII  (2192) 1 site

EagI  (2199) 1 site

NotI  (2199) 1 site

Eco53kI  (2210) 1 site

BseRI  (2212) 1 site

Sticky ends from different BseRI sites may not be compatible. BseRI quickly loses activity at 37°C. Prolonged incubation with BseRI may lead to degradation of the DNA.

SacI  (2212) 1 site

DraIII  (2269) 1 site

Sticky ends from different DraIII sites may not be compatible.

Bsu36I  (2279) 1 site

Sticky ends from different Bsu36I sites may not be compatible.

AmpR 4139 .. 4999  =  861 bp 286 amino acids  =  31.6 kDa    Segment 2:      4139 .. 4930  =  792 bp    263 amino acids  =  28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 4139 .. 4999  =  861 bp 286 amino acids  =  31.6 kDa    Segment 1:  signal sequence      4931 .. 4999  =  69 bp    23 amino acids  =  2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 4139 .. 4999  =  861 bp 286 amino acids  =  31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

baculovirus recombination region (lef2/ORF603) 181 .. 1008  =  828 bp contains ORF603 and part of lef2

baculovirus recombination region (lef2/ORF603) 181 .. 1008  =  828 bp contains ORF603 and part of lef2

baculovirus recombination region (ORF1629) 2624 .. 3329  =  706 bp contains part of ORF1629

baculovirus recombination region (ORF1629) 2624 .. 3329  =  706 bp contains part of ORF1629

ori 3539 .. 4126  =  588 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

ori 3539 .. 4126  =  588 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

Exon 2 1863 .. 2445  =  583 bp

Exon 2 1863 .. 2445  =  583 bp

CMV enhancer 1076 .. 1379  =  304 bp human cytomegalovirus immediate early enhancer

CMV enhancer 1076 .. 1379  =  304 bp human cytomegalovirus immediate early enhancer

CMV promoter 1381 .. 1580  =  200 bp human cytomegalovirus (CMV) immediate early promoter

CMV promoter 1381 .. 1580  =  200 bp human cytomegalovirus (CMV) immediate early promoter

intron 1699 .. 1862  =  164 bp

intron 1699 .. 1862  =  164 bp

Exon 1 1597 .. 1698  =  102 bp

Exon 1 1597 .. 1698  =  102 bp

T7 terminator 2564 .. 2611  =  48 bp transcription terminator for bacteriophage T7 RNA polymerase

T7 terminator 2564 .. 2611  =  48 bp transcription terminator for bacteriophage T7 RNA polymerase

ORF603 343 .. 948  =  606 bp 201 amino acids  =  23.6 kDa Product: baculovirus ORF603 protein

ORF603 343 .. 948  =  606 bp 201 amino acids  =  23.6 kDa Product: baculovirus ORF603 protein

ORF1629 2741 .. 3329  =  589 bp 195 amino acids  =  22.3 kDa Product: baculovirus capsid-associated protein required for viral replication

ORF1629 2741 .. 3329  =  589 bp 195 amino acids  =  22.3 kDa Product: baculovirus capsid-associated protein required for viral replication

lef2 181 .. 305  =  125 bp 40 amino acids  =  4.5 kDa Product: baculovirus late expression factor 2

lef2 181 .. 305  =  125 bp 40 amino acids  =  4.5 kDa Product: baculovirus late expression factor 2

rabbit globin terminator 2443 .. 2554  =  112 bp

rabbit globin terminator 2443 .. 2554  =  112 bp

p10 promoter 1990 .. 2099  =  110 bp baculovirus promoter for expression in insect cells

p10 promoter 1990 .. 2099  =  110 bp baculovirus promoter for expression in insect cells

MCS 2167 .. 2226  =  60 bp multiple cloning site

MCS 2167 .. 2226  =  60 bp multiple cloning site

10xHis 2237 .. 2266  =  30 bp 10 amino acids  =  1.4 kDa Product: 10xHis affinity tag

10xHis 2237 .. 2266  =  30 bp 10 amino acids  =  1.4 kDa Product: 10xHis affinity tag

lac operator 1950 .. 1974  =  25 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

lac operator 1950 .. 1974  =  25 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

Strep-Tag II 2123 .. 2146  =  24 bp 8 amino acids  =  1.1 kDa Product: peptide that binds Strep-Tactin®, an engineered form of streptavidin

Strep-Tag II 2123 .. 2146  =  24 bp 8 amino acids  =  1.1 kDa Product: peptide that binds Strep-Tactin®, an engineered form of streptavidin

T7 promoter 1931 .. 1949  =  19 bp promoter for bacteriophage T7 RNA polymerase

T7 promoter 1931 .. 1949  =  19 bp promoter for bacteriophage T7 RNA polymerase

RBS 2101 .. 2106  =  6 bp ribosome binding site

RBS 2101 .. 2106  =  6 bp ribosome binding site

poly(A) signal 2421 .. 2426  =  6 bp

poly(A) signal 2421 .. 2426  =  6 bp

ATG 2114 .. 2116  =  3 bp 1 amino acid  =  149.2 Da Product: start codon

ATG 2114 .. 2116  =  3 bp 1 amino acid  =  149.2 Da Product: start codon

enterokinase site 2153 .. 2167  =  15 bp 5 amino acids  =  606.5 Da Product: enterokinase recognition and cleavage site

enterokinase site 2153 .. 2167  =  15 bp 5 amino acids  =  606.5 Da Product: enterokinase recognition and cleavage site

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