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pCMV-Cypridina Luc

Control vector for constitutive high-level expression of secreted Cypridina luciferase under control of the CMV promoter.

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pCMV-Cypridina Luc.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Pierce)
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AarI (6556) AflII (5909) DrdI (5576) MauBI (4980) NruI (4907) PvuI (4353) FspI (4205) AfeI (4033) SalI (3873) BstZ17I (3868) DraIII (3578) BsmBI (3399) SpeI (1) NdeI (236) SnaBI (342) Eco53kI (568) SacI (570) BamHI (634) BbsI (655) Bpu10I (808) BstXI (999) BspDI - ClaI (1070) BsrDI (1127) PstI (1462) NheI (1534) BmtI (1538) AleI (1594) BlpI (1600) Bsu36I (1907) KflI - PpuMI (1962) NotI (2309) BseRI (2874) AvrII (2878) PflFI - Tth111I (3049) BsiWI (3063) RsrII (3123) BstEII (3141) EarI (3166) SacII (3221) pCMV-Cypridina Luc 6586 bp
AarI  (6556)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
AflII  (5909)
1 site
C T T A A G G A A T T C
DrdI  (5576)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
MauBI  (4980)
1 site
C G C G C G C G G C G C G C G C
NruI  (4907)
1 site
T C G C G A A G C G C T
PvuI  (4353)
1 site
C G A T C G G C T A G C
FspI  (4205)
1 site
T G C G C A A C G C G T
AfeI  (4033)
1 site
A G C G C T T C G C G A
SalI  (3873)
1 site
G T C G A C C A G C T G
BstZ17I  (3868)
1 site
G T A T A C C A T A T G
DraIII  (3578)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsmBI  (3399)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
SpeI  (1)
1 site
A C T A G T T G A T C A
NdeI  (236)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (342)
1 site
T A C G T A A T G C A T
Eco53kI  (568)
1 site
G A G C T C C T C G A G
SacI  (570)
1 site
G A G C T C C T C G A G
BamHI  (634)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BbsI  (655)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
Bpu10I  (808)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BstXI  (999)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BspDI  (1070)
1 site
A T C G A T T A G C T A
ClaI  (1070)
1 site
A T C G A T T A G C T A
BsrDI  (1127)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PstI  (1462)
1 site
C T G C A G G A C G T C
NheI  (1534)
1 site
G C T A G C C G A T C G
BmtI  (1538)
1 site
G C T A G C C G A T C G
AleI  (1594)
1 site
C A C N N N N G T G G T G N N N N C A C
BlpI  (1600)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
Bsu36I  (1907)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
KflI  (1962)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (1962)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
NotI  (2309)
1 site
G C G G C C G C C G C C G G C G
BseRI  (2874)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
AvrII  (2878)
1 site
C C T A G G G G A T C C
PflFI  (3049)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3049)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsiWI  (3063)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
RsrII  (3123)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BstEII  (3141)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
EarI  (3166)
1 site
C T C T T C N G A G A A G N N N N