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pCMV-Green Renilla Luc

Control vector for constitutive high-level expression of intracellular green Renilla luciferase under control of the CMV promoter.

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pCMV-Green Renilla Luc.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Pierce)
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AarI (5830) EcoRV (5536) ApaI (5196) PspOMI (5192) AflII (5183) DrdI (4850) AlwNI (4543) MauBI (4254) NruI (4181) ScaI (3737) PvuI (3627) FspI (3479) AfeI (3307) SalI (3147) BstZ17I (3142) BsmI (3090) NaeI (2955) SpeI (1) NdeI (236) SnaBI (342) Eco53kI (568) SacI (570) BamHI (634) XcmI (1068) EcoNI (1321) NotI (1583) BseRI (2148) AvrII (2152) TspMI - XmaI (2173) SmaI (2175) PflFI - Tth111I (2323) BsiWI (2337) RsrII (2397) BstEII (2415) SacII (2495) BsmBI (2673) NgoMIV (2953) pCMV-Green Renilla Luc 5860 bp
AarI  (5830)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
EcoRV  (5536)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
ApaI  (5196)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (5192)
1 site
G G G C C C C C C G G G
AflII  (5183)
1 site
C T T A A G G A A T T C
DrdI  (4850)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
AlwNI  (4543)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
MauBI  (4254)
1 site
C G C G C G C G G C G C G C G C
NruI  (4181)
1 site
T C G C G A A G C G C T
ScaI  (3737)
1 site
A G T A C T T C A T G A
PvuI  (3627)
1 site
C G A T C G G C T A G C
FspI  (3479)
1 site
T G C G C A A C G C G T
AfeI  (3307)
1 site
A G C G C T T C G C G A
SalI  (3147)
1 site
G T C G A C C A G C T G
BstZ17I  (3142)
1 site
G T A T A C C A T A T G
BsmI  (3090)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
NaeI  (2955)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
SpeI  (1)
1 site
A C T A G T T G A T C A
NdeI  (236)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (342)
1 site
T A C G T A A T G C A T
Eco53kI  (568)
1 site
G A G C T C C T C G A G
SacI  (570)
1 site
G A G C T C C T C G A G
BamHI  (634)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XcmI  (1068)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
EcoNI  (1321)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
NotI  (1583)
1 site
G C G G C C G C C G C C G G C G
BseRI  (2148)
1 site
G A G G A G ( N ) 8