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pCMV-Red Firefly Luc

Control vector for constitutive high-level expression of intracellular red firefly luciferase under control of the CMV promoter.

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pCMV-Red Firefly Luc.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Pierce)
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AarI - BfuAI - BspMI (6545) EcoRV (6251) AflII (5898) DrdI (5565) MauBI (4969) NruI (4896) ScaI (4452) PvuI (4342) FspI (4194) AfeI (4022) SalI (3862) BstZ17I (3857) BsmI (3805) DraIII (3567) BsmBI (3388) SpeI (2) NdeI (237) SnaBI (343) Eco53kI (569) SacI (571) BamHI (635) BsrDI (768) EcoRI (911) BglII (972) PflMI * (1141) KflI - PpuMI (1321) SbfI (1485) MreI (2051) NotI (2298) BseRI (2863) AvrII (2867) TspMI - XmaI (2888) SmaI (2890) BsiWI (3052) RsrII (3112) BstEII (3130) SacII (3210) pCMV-Red Firefly Luc 6575 bp
AarI  (6545)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
BfuAI  (6545)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (6545)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
EcoRV  (6251)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
AflII  (5898)
1 site
C T T A A G G A A T T C
DrdI  (5565)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
MauBI  (4969)
1 site
C G C G C G C G G C G C G C G C
NruI  (4896)
1 site
T C G C G A A G C G C T
ScaI  (4452)
1 site
A G T A C T T C A T G A
PvuI  (4342)
1 site
C G A T C G G C T A G C
FspI  (4194)
1 site
T G C G C A A C G C G T
AfeI  (4022)
1 site
A G C G C T T C G C G A
SalI  (3862)
1 site
G T C G A C C A G C T G
BstZ17I  (3857)
1 site
G T A T A C C A T A T G
BsmI  (3805)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
DraIII  (3567)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsmBI  (3388)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
SpeI  (2)
1 site
A C T A G T T G A T C A
NdeI  (237)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (343)
1 site
T A C G T A A T G C A T
Eco53kI  (569)
1 site
G A G C T C C T C G A G
SacI  (571)
1 site
G A G C T C C T C G A G
BamHI  (635)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BsrDI  (768)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
EcoRI  (911)
1 site
G A A T T C C T T A A G
BglII  (972)
1 site
A G A T C T T C T A G A
PflMI  (1141)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
KflI  (1321)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (1321)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SbfI  (1485)
1 site
C C T G C A G G G G A C G T C C
MreI  (2051)
1 site
C G C C G G C G G C G G C C G C
NotI  (2298)
1 site
G C G G C C G C C G C C G G C G
BseRI  (2863)
1 site
G A G G A G ( N