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Plasmid Files

pGEM®-luc

Plasmid carrying the firefly luciferase gene flanked by unique restriction sites.

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pGEM-luc.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Promega
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SfiI (17) MscI (11) DraIII (4529) NaeI (4423) NgoMIV (4421) BstAPI (4244) NdeI (4243) AatII (3994) ZraI (3992) XmnI (3671) ScaI (3552) BsaI (3133) AhdI (3072) AlwNI (2595) Eco53kI (25) SacI (27) SalI (35) AccI (36) AbsI - PaeR7I - PspXI - XhoI (41) StuI (47) EcoNI (135) BseRI (200) SgrAI (321) BfuAI - BspMI (351) BspDI - ClaI (386) EcoRV (415) PacI (431) PpuMI (571) XcmI (1019) Bsu36I (1137) BstEII (1141) BsrGI (1259) BsiWI (1595) KasI (1717) NarI (1718) SfoI (1719) PluTI (1721) BamHI (1757) PspOMI (1763) ApaI (1767) NotI (1777) NsiI (1788) HindIII (1790) PciI (2179) pGEM®-luc 4931 bp
SfiI  (17)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
MscI  (11)
1 site
T G G C C A A C C G G T
DraIII  (4529)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
NaeI  (4423)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (4421)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BstAPI  (4244)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
NdeI  (4243)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
AatII  (3994)
1 site
G A C G T C C T G C A G
ZraI  (3992)
1 site
G A C G T C C T G C A G
XmnI  (3671)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (3552)
1 site
A G T A C T T C A T G A
BsaI  (3133)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3072)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2595)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
Eco53kI  (25)
1 site
G A G C T C C T C G A G
SacI  (27)
1 site
G A G C T C C T C G A G
SalI  (35)
1 site
G T C G A C C A G C T G
AccI  (36)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
AbsI  (41)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (41)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (41)
1 site
V C T C G A G B B G A G C T C V
XhoI  (41)
1 site
C T C G A G G A G C T C
StuI  (47)
1 site
A G G C C T T C C G G A
EcoNI  (135)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BseRI  (200)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
SgrAI  (321)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BfuAI  (351)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (351)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BspDI  (386)
1 site
A T C G A T T A G C T A
ClaI  (386)
1 site
A T C G A T T A G C T A
EcoRV  (415)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PacI  (431)
1 site
T T A A T T A A A A T T A A T T
PpuMI  (571)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
XcmI  (1019)
1 site
C C A N N N N N N N N N T G G G G