Plasmid Files

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AarI  (4611) 1 site

Efficient cleavage requires at least two copies of the AarI recognition sequence. Sticky ends from different AarI sites may not be compatible. After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.

BfuAI  (4611) 1 site

Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible. BfuAI is typically used at 50°C, but is 50% active at 37°C.

BspMI  (4611) 1 site

Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible.

BsmBI  (4438) 1 site

Sticky ends from different BsmBI sites may not be compatible.

SpeI  (4425) 1 site

BstZ17I  (4106) 1 site

SacII  (3990) 1 site

Efficient cleavage requires at least two copies of the SacII recognition sequence.

PvuI  (3966) 1 site

Bsu36I  (3952) 1 site

Sticky ends from different Bsu36I sites may not be compatible.

AhdI  (3596) 1 site

The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible.

BstEII  (3521) 1 site

Sticky ends from different BstEII sites may not be compatible. BstEII is typically used at 60°C, but is 50% active at 37°C.

BstXI  (3518) 1 site

Sticky ends from different BstXI sites may not be compatible.

PstI  (3518) 1 site

NotI  (3494) 1 site

AlwNI  (3090) 1 site

Sticky ends from different AlwNI sites may not be compatible.

ApaLI  (2988) 1 site

BciVI  (2877) 1 site

The 1-base overhangs produced by BciVI may be hard to ligate. Sticky ends from different BciVI sites may not be compatible.

AflIII  (2674) 1 site

Sticky ends from different AflIII sites may not be compatible.

PciI  (2674) 1 site

PciI is inhibited by nonionic detergents.

PshAI  (2489) 1 site

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.

SalI  (2424) 1 site

BamHI  (2418) 1 site

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.

MfeI  (2325) 1 site

BglI  (8) 2 sites

Sticky ends from different BglI sites may not be compatible.

SfiI  (8) 2 sites

Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible.

Eco53kI  (23) 1 site

SacI  (25) 1 site

NheI  (27) 1 site

BmtI  (31) 1 site

AbsI  (33) 1 site

PaeR7I  (33) 1 site

PaeR7I does not recognize the sequence CTCTCGAG.

PspXI  (33) 1 site

XhoI  (33) 1 site

EcoRV  (41) 1 site

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.

BglII  (45) 1 site

BglI  (401) 2 sites

Sticky ends from different BglI sites may not be compatible.

SfiI  (401) 2 sites

Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible.

StuI  (447) 1 site

AvrII  (448) 1 site

HindIII  (464) 1 site

PspOMI  (525) 1 site

ApaI  (529) 1 site

ApaI can be used between 25°C and 37°C.

MreI  (562) 1 site

SgrAI  (562) 1 site

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.

BsrGI  (989) 1 site

BsrGI is typically used at 37°C, but is even more active at 60°C.

BbvCI  (1210) 1 site

KasI  (1446) 1 site

NarI  (1447) 1 site

Efficient cleavage requires at least two copies of the NarI recognition sequence.

SfoI  (1448) 1 site

PluTI  (1450) 1 site

Efficient cleavage requires at least two copies of the PluTI recognition sequence.

BlpI  (1453) 1 site

Sticky ends from different BlpI sites may not be compatible.

AgeI  (1630) 1 site

AgeI quickly loses activity at 37°C, but can be used at 25°C for long incubations.

DraIII  (1645) 1 site

Sticky ends from different DraIII sites may not be compatible.

BpmI  (1879) 1 site

Efficient cleavage requires at least two copies of the BpmI recognition sequence. Sticky ends from different BpmI sites may not be compatible. After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility. BpmI quickly loses activity at 37°C.

XbaI  (2156) 1 site

FseI  (2175) 1 site

FseI gradually loses activity when stored at -20°C.

ApoI  (2261) 1 site

ApoI is typically used at 50°C, but is 50% active at 37°C.

PsiI  (2296) 1 site

RVprimer3 20-mer  /  50% GC 1 binding site 4590 .. 4609  =  20 annealed bases Tm  =  54°C

RVprimer4 20-mer  /  65% GC 1 binding site 2475 .. 2494  =  20 annealed bases Tm  =  61°C

luciferase 499 .. 2151  =  1653 bp 550 amino acids  =  60.6 kDa Product: firefly luciferase synthetic luc2 version of the luciferase gene

luciferase 499 .. 2151  =  1653 bp 550 amino acids  =  60.6 kDa Product: firefly luciferase synthetic luc2 version of the luciferase gene

AmpR 3523 .. 4383  =  861 bp 286 amino acids  =  31.6 kDa    Segment 2:      3523 .. 4314  =  792 bp    263 amino acids  =  28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 3523 .. 4383  =  861 bp 286 amino acids  =  31.6 kDa    Segment 1:  signal sequence      4315 .. 4383  =  69 bp    23 amino acids  =  2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 3523 .. 4383  =  861 bp 286 amino acids  =  31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

ori 2735 .. 3323  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

ori 2735 .. 3323  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

SV40 promoter 106 .. 463  =  358 bp SV40 enhancer and early promoter

SV40 promoter 106 .. 463  =  358 bp SV40 enhancer and early promoter

SV40 poly(A) signal 2195 .. 2316  =  122 bp SV40 polyadenylation signal

SV40 poly(A) signal 2195 .. 2316  =  122 bp SV40 polyadenylation signal

pause site 4550 .. 4641  =  92 bp RNA polymerase II transcriptional pause signal from the human α2 globin gene

pause site 4550 .. 4641  =  92 bp RNA polymerase II transcriptional pause signal from the human α2 globin gene

poly(A) signal 4488 .. 4536  =  49 bp synthetic polyadenylation signal

poly(A) signal 4488 .. 4536  =  49 bp synthetic polyadenylation signal

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