Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
BfuAI (4611) 1 site
ACCTGC(N)4TGGACG(N)4(N)4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI (4611) 1 site
ACCTGC(N)4TGGACG(N)4(N)4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BsmBI (4438) 1 site
CGTCTCNGCAGAGN(N)4
Sticky ends from different BsmBI sites may not be compatible.
SpeI (4425) 1 site
ACTAGTTGATCA
BstZ17I (4106) 1 site
GTATACCATATG
SacII (3990) 1 site
CCGCGGGGCGCC
Efficient cleavage requires at least two copies of the SacII recognition sequence.
PvuI (3966) 1 site
CGATCGGCTAGC
Bsu36I (3952) 1 site
CCTNAGGGGANTCC
Sticky ends from different Bsu36I sites may not be compatible.
AhdI (3596) 1 site
GACNNNNNGTCCTGNNNNNCAG
The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible.
BstEII (3521) 1 site
GGTNACCCCANTGG
Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C.
BstXI (3518) 1 site
CCANNNNNNTGGGGTNNNNNNACC
Sticky ends from different BstXI sites may not be compatible.
PstI (3518) 1 site
CTGCAGGACGTC
NotI (3494) 1 site
GCGGCCGCCGCCGGCG
AlwNI (3090) 1 site
CAGNNNCTGGTCNNNGAC
Sticky ends from different AlwNI sites may not be compatible.
ApaLI (2988) 1 site
GTGCACCACGTG
BciVI (2877) 1 site
GTATCC(N)5NCATAGG(N)5
The 1-base overhangs produced by BciVI may be hard to ligate.Sticky ends from different BciVI sites may not be compatible.
AflIII (2674) 1 site
ACRYGTTGYRCA
Sticky ends from different AflIII sites may not be compatible.
PciI (2674) 1 site
ACATGTTGTACA
PciI is inhibited by nonionic detergents.
PshAI (2489) 1 site
GACNNNNGTCCTGNNNNCAG
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
SalI (2424) 1 site
GTCGACCAGCTG
BamHI (2418) 1 site
GGATCCCCTAGG
After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
MfeI (2325) 1 site
CAATTGGTTAAC
BglI (8) 2 sites
GCCNNNNNGGCCGGNNNNNCCG
Sticky ends from different BglI sites may not be compatible.
SfiI (8) 2 sites
GGCCNNNNNGGCCCCGGNNNNNCCGG
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
Eco53kI (23) 1 site
GAGCTCCTCGAG
SacI (25) 1 site
GAGCTCCTCGAG
NheI (27) 1 site
GCTAGCCGATCG
BmtI (31) 1 site
GCTAGCCGATCG
AbsI (33) 1 site
CCTCGAGGGGAGCTCC
PaeR7I (33) 1 site
CTCGAGGAGCTC
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI (33) 1 site
VCTCGAGBBGAGCTCV
XhoI (33) 1 site
CTCGAGGAGCTC
EcoRV (41) 1 site
GATATCCTATAG
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BglII (45) 1 site
AGATCTTCTAGA
BglI (401) 2 sites
GCCNNNNNGGCCGGNNNNNCCG
Sticky ends from different BglI sites may not be compatible.
SfiI (401) 2 sites
GGCCNNNNNGGCCCCGGNNNNNCCGG
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
StuI (447) 1 site
AGGCCTTCCGGA
AvrII (448) 1 site
CCTAGGGGATCC
HindIII (464) 1 site
AAGCTTTTCGAA
PspOMI (525) 1 site
GGGCCCCCCGGG
ApaI (529) 1 site
GGGCCCCCCGGG
ApaI can be used between 25°C and 37°C.
MreI (562) 1 site
CGCCGGCGGCGGCCGC
SgrAI (562) 1 site
CRCCGGYGGYGGCCRC
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BsrGI (989) 1 site
TGTACAACATGT
BsrGI is typically used at 37°C, but is even more active at 60°C.
BbvCI (1210) 1 site
CCTCAGCGGAGTCG
KasI (1446) 1 site
GGCGCCCCGCGG
NarI (1447) 1 site
GGCGCCCCGCGG
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI (1448) 1 site
GGCGCCCCGCGG
PluTI (1450) 1 site
GGCGCCCCGCGG
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BlpI (1453) 1 site
GCTNAGCCGANTCG
Sticky ends from different BlpI sites may not be compatible.
AgeI (1630) 1 site
ACCGGTTGGCCA
DraIII (1645) 1 site
CACNNNGTGGTGNNNCAC
Sticky ends from different DraIII sites may not be compatible.
BpmI (1879) 1 site
CTGGAG(N)14NNGACCTC(N)14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.BpmI quickly loses activity at 37°C.
XbaI (2156) 1 site
TCTAGAAGATCT
FseI (2175) 1 site
GGCCGGCCCCGGCCGG
FseI gradually loses activity when stored at -20°C.
ApoI (2261) 1 site
RAATTYYTTAAR
ApoI is typically used at 50°C, but is 50% active at 37°C.
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