pGL4.75[hRluc CMV]

Co-transfection vector for expressing Renilla luciferase under control of the strong CMV promoter.

Sequence Author: Promega

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Luciferase Vectors | More Plasmid Sets
No matches
BsgI (4279) AarI - BfuAI - BspMI (4251) RVprimer3 (4230 .. 4249) BsmBI (4078) BstZ17I (3746) SacII (3630) PvuI (3606) AhdI (3236) BstEII (3161) BstXI - PstI (3158) AleI (3156) NotI (3134) BspHI (3034) AlwNI (2730) BaeGI - Bme1580I (2632) ApaLI (2628) BciVI (2517) BssSI (2487) DrdI (2422) NspI (2318) AflIII - PciI (2314) BspQI - SapI (2198) AfeI (2190) BbsI (2143) SfiI (8) BsrGI (109) AseI (173) NdeI (400) BtgZI (500) BsaAI - SnaBI (506) Eco53kI (732) SacI (734) SfiI (818) AvaI - BmeT110I - BsoBI (880) BstAPI - DraIII (1047) NsiI (1078) PfoI * (1139) EcoRV (1344) XmnI (1393) BsaI (1399) NruI (1520) PflFI - Tth111I (1555) PasI (1633) XbaI (1796) FseI (1815) ApoI (1901) PsiI (1936) HpaI (1956) MfeI (1965) BamHI (2058) SalI (2064) PshAI (2129) RVprimer4 (2115 .. 2134) pGL4.75[hRluc/CMV] 4281 bp
BsgI  (4279)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AarI  (4251)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Cleavage may be enhanced when more than one copy of the AarI recognition sequence is present.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
BfuAI  (4251)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (4251)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BsmBI  (4078)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
BstZ17I  (3746)
1 site
G T A T A C C A T A T G
SacII  (3630)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PvuI  (3606)
1 site
C G A T C G G C T A G C
AhdI  (3236)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BstEII  (3161)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BstXI  (3158)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
PstI  (3158)
1 site
C T G C A G G A C G T C
AleI  (3156)
1 site
C A C N N N N G T G G T G N N N N C A C
NotI  (3134)
1 site
G C G G C C G C C G C C G G C G
BspHI  (3034)
1 site
T C A T G A A G T A C T
AlwNI  (2730)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BaeGI  (2632)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (2632)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
ApaLI  (2628)
1 site
G T G C A C C A C G T G
BciVI  (2517)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BssSI  (2487)
1 site
C A C G A G G T G C T C
DrdI  (2422)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
NspI  (2318)
1 site
R C A T G Y Y G T A C R
AflIII  (2314)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (2314)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (2198)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2198)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AfeI  (2190)
1 site
A G C G C T T C G C G A
BbsI  (2143)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
SfiI  (8)
2 sites
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BsrGI  (109)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
AseI  (173)
1 site
A T T A A T T A A T T A
NdeI  (400)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BtgZI  (500)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BsaAI  (506)
1 site
Y A C G T R R T G C A Y
SnaBI  (506)
1 site
T A C G T A A T G C A T
Eco53kI  (732)
1 site
G A G C T C C T C G A G
SacI  (734)
1 site
G A G C T C C T C G A G
SfiI  (818)
2 sites
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AvaI  (880)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BmeT110I  (880)
1 site
C Y C G R G G R G C Y C
BsoBI  (880)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
BstAPI  (1047)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
DraIII  (1047)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
NsiI  (1078)
1 site
A T G C A T T A C G T A
PfoI  (1139)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
EcoRV  (1344)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
XmnI  (1393)
1 site
G A A N N N N T T C C T T N N N N A A G
BsaI  (1399)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
NruI  (1520)
1 site
T C G C G A A G C G C T
PflFI  (1555)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1555)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PasI  (1633)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
XbaI  (1796)
1 site
T C T A G A A G A T C T
FseI  (1815)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
ApoI  (1901)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
PsiI  (1936)
1 site
T T A T A A A A T A T T
HpaI  (1956)
1 site
G T T A A C C A A T T G
MfeI  (1965)
1 site
C A A T T G G T T A A C
BamHI  (2058)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SalI  (2064)
1 site
G T C G A C C A G C T G
PshAI  (2129)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
RVprimer3
20-mer  /  50% GC
1 binding site
4230 .. 4249  =  20 annealed bases
Tm  =  54°C
RVprimer4
20-mer  /  65% GC
1 binding site
2115 .. 2134  =  20 annealed bases
Tm  =  62°C
hRluc
859 .. 1794  =  936 bp
311 amino acids  =  36.0 kDa
Product: Renilla luciferase
human codon-optimized
hRluc
859 .. 1794  =  936 bp
311 amino acids  =  36.0 kDa
Product: Renilla luciferase
human codon-optimized
AmpR
3163 .. 4023  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3163 .. 3954  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3163 .. 4023  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3955 .. 4023  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3163 .. 4023  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
2375 .. 2963  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2375 .. 2963  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
227 .. 530  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
227 .. 530  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
531 .. 734  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
531 .. 734  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
1835 .. 1956  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1835 .. 1956  =  122 bp
SV40 polyadenylation signal
pause site
4190 .. 4281  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
pause site
4190 .. 4281  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
poly(A) signal
4128 .. 4176  =  49 bp
synthetic polyadenylation signal
poly(A) signal
4128 .. 4176  =  49 bp
synthetic polyadenylation signal
ORF:  859 .. 1794  =  936 bp
ORF:  311 amino acids  =  36.0 kDa
ORF:  3293 .. 3559  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  1921 .. 2163  =  243 bp
ORF:  80 amino acids  =  9.0 kDa
ORF:  3163 .. 4023  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download pGL4.75[hRluc CMV].dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.