pLVX-MetLuc Control

Lentiviral vector for constitutive expression of secreted Metridia luciferase.

Sequence Author: Clontech (TaKaRa)

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SgrDI (8401) SspI (8284) PvuI (7850) FspI (7702) DrdI (6695) AflIII - PciI (6587) Bsu36I (5409) PflMI (4930) CsiI - SexAI * (4643) BstEII (4210) NruI * (833) FseI (1150) MfeI (1189) BbvCI (1424) KflI (1934) cPPT/CTS BspDI - ClaI (2180) NdeI (2374) SnaBI (2480) AfeI (2798) PaeR7I - XhoI (2815) BstBI (2829) EcoRI (2831) PspOMI (2855) ApaI (2859) BamHI (2862) BclI * (3040) XcmI (3080) BsrGI (3241) PaqCI (3244) SphI (3595) AgeI (3666) SpeI (3801) BlpI (3829) PasI (4125) BsiWI (4132) RsrII (4192) pLVX-MetLuc Control 8721 bp
SgrDI  (8401)
1 site
C G T C G A C G G C A G C T G C
SspI  (8284)
1 site
A A T A T T T T A T A A
PvuI  (7850)
1 site
C G A T C G G C T A G C
FspI  (7702)
1 site
T G C G C A A C G C G T
DrdI  (6695)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
AflIII  (6587)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (6587)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
Bsu36I  (5409)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PflMI  (4930)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
CsiI  (4643)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (4643)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BstEII  (4210)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
NruI  (833)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
FseI  (1150)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
MfeI  (1189)
1 site
C A A T T G G T T A A C
BbvCI  (1424)
1 site
C C T C A G C G G A G T C G
KflI  (1934)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
BspDI  (2180)
1 site
A T C G A T T A G C T A
ClaI  (2180)
1 site
A T C G A T T A G C T A
NdeI  (2374)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (2480)
1 site
T A C G T A A T G C A T
AfeI  (2798)
1 site
A G C G C T T C G C G A
PaeR7I  (2815)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2815)
1 site
C T C G A G G A G C T C
BstBI  (2829)
1 site
T T C G A A A A G C T T
EcoRI  (2831)
1 site
G A A T T C C T T A A G
PspOMI  (2855)
1 site
G G G C C C C C C G G G
ApaI  (2859)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BamHI  (2862)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BclI  (3040)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
XcmI  (3080)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsrGI  (3241)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
PaqCI  (3244)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
SphI  (3595)
1 site
G C A T G C C G T A C G
AgeI  (3666)
1 site
A C C G G T T G G C C A
SpeI  (3801)
1 site
A C T A G T T G A T C A
BlpI  (3829)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
PasI  (4125)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BsiWI  (4132)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
RsrII  (4192)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AmpR
7407 .. 8267  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   7407 .. 8198  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7407 .. 8267  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   8199 .. 8267  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7407 .. 8267  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
MetLuc
2880 .. 3539  =  660 bp
219 amino acids  =  23.9 kDa
2 segments
   Segment 1:  signal sequence  
   2880 .. 2930  =  51 bp
   17 amino acids  =  1.9 kDa
Product: secreted Metridia luciferase
human codon-optimized
MetLuc
2880 .. 3539  =  660 bp
219 amino acids  =  23.9 kDa
2 segments
   Segment 2:  
   2931 .. 3539  =  609 bp
   202 amino acids  =  22.0 kDa
Product: secreted Metridia luciferase
human codon-optimized
MetLuc
2880 .. 3539  =  660 bp
219 amino acids  =  23.9 kDa
2 segments
Product: secreted Metridia luciferase
human codon-optimized
3' LTR
1 .. 634  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
1 .. 634  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
5484 .. 6117  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
5484 .. 6117  =  634 bp
3' long terminal repeat (LTR) from HIV-1
PuroR
4076 .. 4675  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
4076 .. 4675  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
WPRE
4689 .. 5277  =  589 bp
woodchuck posttranscriptional regulatory element
WPRE
4689 .. 5277  =  589 bp
woodchuck posttranscriptional regulatory element
ori
6648 .. 7236  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6648 .. 7236  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
PGK promoter
3556 .. 4055  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
3556 .. 4055  =  500 bp
mouse phosphoglycerate kinase 1 promoter
CMV enhancer
2201 .. 2504  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2201 .. 2504  =  304 bp
human cytomegalovirus immediate early enhancer
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
CMV promoter
2505 .. 2708  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
2505 .. 2708  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
8420 .. 8554  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
8420 .. 8554  =  135 bp
SV40 polyadenylation signal
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
2028 .. 2144  =  117 bp
central polypurine tract and central termination sequence of HIV-1 (lacking the first T)
cPPT/CTS
2028 .. 2144  =  117 bp
central polypurine tract and central termination sequence of HIV-1 (lacking the first T)
AmpR promoter
8268 .. 8372  =  105 bp
AmpR promoter
8268 .. 8372  =  105 bp
ORF:  4792 .. 5088  =  297 bp
ORF:  98 amino acids  =  10.2 kDa
ORF:  7537 .. 7803  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1181 .. 2032  =  852 bp
ORF:  283 amino acids  =  32.7 kDa
ORF:  3836 .. 4675  =  840 bp
ORF:  279 amino acids  =  30.1 kDa
ORF:  2880 .. 3539  =  660 bp
ORF:  219 amino acids  =  23.9 kDa
ORF:  4779 .. 5306  =  528 bp
ORF:  175 amino acids  =  19.0 kDa
ORF:  1015 .. 1476  =  462 bp
ORF:  153 amino acids  =  16.6 kDa
ORF:  7407 .. 8267  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  2822 .. 3247  =  426 bp
ORF:  141 amino acids  =  15.2 kDa
ORF:  3266 .. 3523  =  258 bp
ORF:  85 amino acids  =  9.4 kDa
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