Resources
Plasmid Files

pLVX-MetLuc Reporter

Lentiviral vector for measuring the activity of promoter and enhancer sequences using secreted Metridia luciferase.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pLVX-MetLuc Reporter Sequence and MappLVX-MetLuc Reporter.dna
Map and Sequence File   
Sequence Author:  Clontech
Download Free Trial Get SnapGene Viewer

 SgrDI (7768) SspI (7651) PvuI (7217) FspI (7069) DrdI (6062) AflIII - PciI (5954) BmtI (5570) NheI (5566) Bsu36I (4776) PflMI (4297) NruI * (833) FseI (1150) MfeI (1189) BbvCI (1424) KflI (1934) BstBI (2196) EcoRI (2198) PspOMI (2222) ApaI (2226) BamHI (2229) BclI * (2407) XcmI (2447) BsrGI (2608) AarI (2611) SphI (2962) AgeI (3033) SpeI (3168) BlpI (3196) PasI (3492) BsiWI (3499) RsrII (3559) BstEII (3577) SexAI * (4010) pLVX-MetLuc Reporter 8088 bp
SgrDI  (7768)
1 site
C G T C G A C G G C A G C T G C
SspI  (7651)
1 site
A A T A T T T T A T A A
PvuI  (7217)
1 site
C G A T C G G C T A G C
FspI  (7069)
1 site
T G C G C A A C G C G T
DrdI  (6062)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
AflIII  (5954)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (5954)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BmtI  (5570)
1 site
G C T A G C C G A T C G
NheI  (5566)
1 site
G C T A G C C G A T C G
Bsu36I  (4776)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PflMI  (4297)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
NruI  (833)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
FseI  (1150)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
MfeI  (1189)
1 site
C A A T T G G T T A A C
BbvCI  (1424)
1 site
C C T C A G C G G A G T C G
KflI  (1934)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
BstBI  (2196)
1 site
T T C G A A A A G C T T
EcoRI  (2198)
1 site
G A A T T C C T T A A G
PspOMI  (2222)
1 site
G G G C C C C C C G G G
ApaI  (2226)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BamHI  (2229)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
BclI  (2407)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
XcmI  (2447)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsrGI  (2608)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
AarI  (2611)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
SphI  (2962)
1 site
G C A T G C C G T A C G
AgeI  (3033)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
SpeI  (3168)
1 site
A C T A G T T G A T C A
BlpI  (3196)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
PasI  (3492)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BsiWI  (3499)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
RsrII  (3559)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BstEII  (3577)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
SexAI  (4010)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
AmpR
6774 .. 7634  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   6774 .. 7565  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
6774 .. 7634  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   7566 .. 7634  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
6774 .. 7634  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
MetLuc
2247 .. 2906  =  660 bp
219 amino acids  =  23.9 kDa
   Segment 1:  signal sequence  
   2247 .. 2297  =  51 bp
   17 amino acids  =  1.9 kDa
Product: secreted Metridia luciferase
human codon-optimized
MetLuc
2247 .. 2906  =  660 bp
219 amino acids  =  23.9 kDa
   Segment 2:  
   2298 .. 2906  =  609 bp
   202 amino acids  =  22.0 kDa
Product: secreted Metridia luciferase
human codon-optimized
MetLuc
2247 .. 2906  =  660 bp
219 amino acids  =  23.9 kDa
2 segments
Product: secreted Metridia luciferase
human codon-optimized
3' LTR
1 .. 634  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
1 .. 634  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
4851 .. 5484  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
4851 .. 5484  =  634 bp
3' long terminal repeat (LTR) from HIV-1
PuroR
3443 .. 4042  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
3443 .. 4042  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
WPRE
4056 .. 4644  =  589 bp
woodchuck posttranscriptional regulatory element
WPRE
4056 .. 4644  =  589 bp
woodchuck posttranscriptional regulatory element
ori
6015 .. 6603  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
6015 .. 6603  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
PGK promoter
2923 .. 3422  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
2923 .. 3422  =  500 bp
mouse phosphoglycerate kinase 1 promoter
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
SV40 poly(A) signal
7787 .. 7921  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
7787 .. 7921  =  135 bp
SV40 polyadenylation signal
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus
type 1
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus
type 1
cPPT/CTS
2028 .. 2144  =  117 bp
central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)
cPPT/CTS
2028 .. 2144  =  117 bp
central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)
AmpR promoter
7635 .. 7739  =  105 bp
AmpR promoter
7635 .. 7739  =  105 bp
MCS
2195 .. 2234  =  40 bp
multiple cloning site
MCS
2195 .. 2234  =  40 bp
multiple cloning site
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter