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pNL1.3.CMV[secNluc/CMV]

Co-transfection vector for expressing secreted NanoLuc® luciferase under control of the strong CMV promoter.

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pNL1.3.CMV[secNluc CMV].dna
Map and Sequence File:    Download    Open   
Sequence Author:  Promega
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AarI - BfuAI - BspMI (3915) RVprimer3 (3894 .. 3913) BsmBI (3742) BstZ17I (3410) SacII (3294) PvuI (3270) Bsu36I (3256) AhdI (2900) BstEII (2825) BstXI - PstI (2822) AleI (2820) NotI (2798) BspHI (2698) AlwNI (2394) BaeGI - Bme1580I (2296) ApaLI (2292) BciVI (2181) NspI (1982) SfiI (8) BsrGI (109) AseI (173) NdeI (400) BsaAI - SnaBI (506) NcoI - StyI (526) Eco53kI (732) BanII - SacI (734) SfiI (818) PasI (903) PflFI - Tth111I (1006) EcoNI (1015) AvaI - BsoBI (1048) BmeT110I (1049) Bpu10I (1084) PpuMI - SanDI (1334) XbaI (1460) FseI (1479) AanI - PsiI (1600) HpaI (1620) MfeI (1629) BamHI (1722) SalI (1728) PshAI (1793) RVprimer4 (1779 .. 1798) AfeI (1854) BspQI - SapI (1862) AflIII - PciI (1978) pNL1.3.CMV[secNluc/CMV] 3945 bp
AarI  (3915)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
BfuAI  (3915)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (3915)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BsmBI  (3742)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BstZ17I  (3410)
1 site
G T A T A C C A T A T G
SacII  (3294)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PvuI  (3270)
1 site
C G A T C G G C T A G C
Bsu36I  (3256)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
AhdI  (2900)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BstEII  (2825)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BstXI  (2822)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
PstI  (2822)
1 site
C T G C A G G A C G T C
AleI  (2820)
1 site
C A C N N N N G T G G T G N N N N C A C
NotI  (2798)
1 site
G C G G C C G C C G C C G G C G
BspHI  (2698)
1 site
T C A T G A A G T A C T
AlwNI  (2394)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BaeGI  (2296)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (2296)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
ApaLI  (2292)
1 site
G T G C A C C A C G T G
BciVI  (2181)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
NspI  (1982)
1 site
R C A T G Y Y G T A C R
SfiI  (8)
2 sites
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BsrGI  (109)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
AseI  (173)
1 site
A T T A A T T A A T T A
NdeI  (400)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BsaAI  (506)
1 site
Y A C G T R R T G C A Y
SnaBI  (506)
1 site
T A C G T A A T G C A T
NcoI  (526)
1 site
C C A T G G G G T A C C
StyI  (526)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
Eco53kI  (732)
1 site
G A G C T C C T C G A G
BanII  (734)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (734)
1 site
G A G C T C C T C G A G
SfiI  (818)
2 sites
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
PasI  (903)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PflFI  (1006)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1006)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
EcoNI  (1015)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
AvaI  (1048)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (1048)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
BmeT110I  (1049)
1 site
C Y C G R G G R G C Y C
Bpu10I  (1084)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PpuMI  (1334)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SanDI  (1334)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
XbaI  (1460)
1 site
T C T A G A A G A T C T
FseI  (1479)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
AanI  (1600)
1 site
T T A T A A A A T A T T
PsiI  (1600)
1 site
T T A T A A A A T A T T
HpaI  (1620)
1 site
G T T A A C C A A T T G
MfeI  (1629)
1 site
C A A T T G G T T A A C
BamHI  (1722)
1 site