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Plasmid Files

pSELECT-zeo-Lucia

Mammalian vector for the expression of secreted Lucia® luciferase.

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pSELECT-zeo-Lucia Sequence and MappSELECT-zeo-Lucia.dna
Map and Sequence File   
Sequence Author:  InvivoGen
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 NotI (2) BciVI (3224) BssS α I (3194) DrdI (3129) NspI (3025) AflIII - PciI (3021) PstI - SbfI (3008) NdeI (2826) BsaAI - SnaBI (2722) SpeI (2592) SacI (2498) Eco53kI (2496) BsmBI (2469) AseI (2438) SfiI (2384) BssHII - MauBI (2341) BsrBI (2327) SmaI (2291) TspMI - XmaI (2289) SgrAI (2263) BmgBI (2247) SexAI * (2213) NmeAIII (2133) FseI (2109) DraIII (2031) BseRI (2008) poly(A) signal AsiSI - PvuI (176) AclI (370) PvuII (407) HindIII (411) KasI (701) NarI (702) SfoI (703) PluTI (705) AgeI (718) BstEII (721) NcoI (726) BbvCI (771) BbsI (804) BglII (841) EcoRV (1138) NheI (1360) BmtI (1364) Bts α I (1426) PsiI (1480) HpaI (1500) EcoRI (1594) BspQI - EarI - SapI (1783) SspI (1835) pSELECT-zeo-Lucia 3769 bp
NotI  (2)
1 site
G C G G C C G C C G C C G G C G
BciVI  (3224)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BssSαI  (3194)
1 site
C A C G A G G T G C T C
DrdI  (3129)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
NspI  (3025)
1 site
R C A T G Y Y G T A C R
AflIII  (3021)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (3021)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PstI  (3008)
1 site
C T G C A G G A C G T C
SbfI  (3008)
1 site
C C T G C A G G G G A C G T C C
NdeI  (2826)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
BsaAI  (2722)
1 site
Y A C G T R R T G C A Y
SnaBI  (2722)
1 site
T A C G T A A T G C A T
SpeI  (2592)
1 site
A C T A G T T G A T C A
SacI  (2498)
1 site
G A G C T C C T C G A G
Eco53kI  (2496)
1 site
G A G C T C C T C G A G
BsmBI  (2469)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
AseI  (2438)
1 site
A T T A A T T A A T T A
SfiI  (2384)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BssHII  (2341)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
MauBI  (2341)
1 site
C G C G C G C G G C G C G C G C
BsrBI  (2327)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
SmaI  (2291)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (2289)
1 site
C C C G G G G G G C C C
XmaI  (2289)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
SgrAI  (2263)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
BmgBI  (2247)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
SexAI  (2213)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
NmeAIII  (2133)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
FseI  (2109)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
DraIII  (2031)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BseRI  (2008)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
AsiSI  (176)
1 site
G C G A T C G C C G C T A G C G
PvuI  (176)
1 site
C G A T C G G C T A G C
AclI  (370)
1 site
A A C G T T T T G C A A
PvuII  (407)
1 site
C A G C T G G T C G A C
HindIII  (411)
1 site
A A G C T T T T C G A A
KasI  (701)
1 site
G G C G C C C C G C G G
NarI  (702)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
SfoI  (703)
1 site
G G C G C C C C G C G G
PluTI  (705)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
AgeI  (718)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BstEII  (721)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
NcoI  (726)
1 site
C C A T G G G G T A C C
BbvCI  (771)
1 site
C C T C A G C G G A G T C G
BbsI  (804)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BglII  (841)
1 site
A G A T C T T C T A G A
EcoRV  (1138)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
NheI  (1360)
1 site
G C T A G C C G A T C G
BmtI  (1364)
1 site
G C T A G C C G A T C G
BtsαI  (1426)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
PsiI  (1480)
1 site
T T A T A A A A T A T T
HpaI  (1500)
1 site
G T T A A C C A A T T G
EcoRI  (1594)
1 site
G A A T T C C T T A A G
BspQI  (1783)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
EarI  (1783)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
SapI  (1783)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
SspI  (1835)
1 site
A A T A T T T T A T A A
Lucia®
728 .. 1357  =  630 bp
209 amino acids  =  22.8 kDa
Product: secreted coelenterazine-utilizing luciferase
synthetic gene based on luciferases from marine
copepods
Lucia®
728 .. 1357  =  630 bp
209 amino acids  =  22.8 kDa
Product: secreted coelenterazine-utilizing luciferase
synthetic gene based on luciferases from marine
copepods
ori
3082 .. 3670  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3082 .. 3670  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
EF-1α/HTLV composite promoter
178 .. 688  =  511 bp
   Segment 1:  EF-1α segment  
   178 .. 407  =  230 bp
EF-1α/HTLV composite promoter
178 .. 688  =  511 bp
   Segment 2:  
   408 .. 419  =  12 bp
EF-1α/HTLV composite promoter
178 .. 688  =  511 bp
   Segment 3:  HTLV segment  
   420 .. 688  =  269 bp
EF-1α/HTLV composite promoter
178 .. 688  =  511 bp
3 segments
β-globin poly(A)
1600 .. 1994  =  395 bp
human β-globin polyadenylation signal
β-globin poly(A)
1600 .. 1994  =  395 bp
human β-globin polyadenylation signal
BleoR
2008 .. 2382  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and
Zeocin™
BleoR
2008 .. 2382  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and
Zeocin™
CMV enhancer
2699 .. 3002  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2699 .. 3002  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
2495 .. 2698  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
2495 .. 2698  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 poly(A) signal
1379 .. 1500  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1379 .. 1500  =  122 bp
SV40 polyadenylation signal
pause site
68 .. 159  =  92 bp
RNA polymerase II transcriptional pause signal from
the human α2 globin gene
pause site
68 .. 159  =  92 bp
RNA polymerase II transcriptional pause signal from
the human α2 globin gene
poly(A) signal
9 .. 57  =  49 bp
synthetic polyadenylation signal
poly(A) signal
9 .. 57  =  49 bp
synthetic polyadenylation signal
T7 promoter
2392 .. 2410  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
2392 .. 2410  =  19 bp
promoter for bacteriophage T7 RNA polymerase
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