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Plasmid Files

pSF-CMV-FLuc

Mammalian vector for strong constitutive expression of firefly luciferase.

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pSF-CMV-FLuc.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Oxford Genetics
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PmeI (5817) AhdI (5580) BsaI (5514) PmeI (4696) PpuMI - SanDI (4630) AscI - BssHII (4539) FseI (4393) SwaI (4287) AlwNI (3814) ApaLI (3712) SwaI (3394) PacI (3268) PstI - SbfI (3136) AsiSI - PvuI (5) BglII (232) SnaBI (566) BglII (816) EagI - NotI (858) HindIII (869) Eco53kI (879) BanII - SacI (881) EcoRI (885) Acc65I (897) KpnI (901) NcoI (905) BbsI (965) PvuII (981) ScaI (1072) BsrGI (1397) AleI (1405) FspI (1729) KasI (1854) NarI (1855) SfoI (1856) PluTI (1858) BlpI (1861) AgeI (2038) DraIII (2053) SexAI * (2246) XbaI (2555) BseRI - BsgI (2558) BspDI - ClaI (2595) BamHI (2604) StuI (2614) NheI (2620) BmtI (2624) PsiI (2770) MfeI (2799) pSF-CMV-FLuc 5936 bp
PmeI  (5817)
2 sites
G T T T A A A C C A A A T T T G
AhdI  (5580)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (5514)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PmeI  (4696)
2 sites
G T T T A A A C C A A A T T T G
PpuMI  (4630)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SanDI  (4630)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
AscI  (4539)
1 site
G G C G C G C C C C G C G C G G
BssHII  (4539)
1 site
G C G C G