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pSF-pA-PromMCS-RLuc

Vector for low-background measurements of promoter activity using Renilla luciferase.

To see this sequence with restriction sites, features, and translations, please download
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pSF-pA-PromMCS-RLuc.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Oxford Genetics
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PmeI (4649) RsrII (4342) BsrDI (4059) PflFI - Tth111I (3944) PmeI (3601) PpuMI - SanDI (3535) AscI (3444) FseI (3298) NaeI (3296) NgoMIV (3294) SwaI (3192) BspHI (3023) AlwNI (2719) AsiSI - PvuI (5) BglII (232) ScaI (299) AarI (364) SalI (395) AccI (396) SpeI (403) TspMI - XmaI (411) SmaI (413) PspOMI (419) ApaI (423) BstBI (428) BglII (435) EagI - NotI (477) HindIII (488) Eco53kI (498) SacI (500) EcoRI (504) Acc65I (516) KpnI (520) BstAPI (714) AanI (818) Bpu10I (1174) BseRI - BsgI (1463) BspDI - ClaI (1500) BamHI (1509) StuI (1519) NheI (1525) BmtI (1529) AanI (1675) HpaI (1695) MfeI (1704) BglI (1864) PstI - SbfI (2041) BstEII (2147) PacI (2173) SwaI (2299) pSF-pA-PromMCS-RLuc 4768 bp
PmeI  (4649)
2 sites
G T T T A A A C C A A A T T T G
RsrII  (4342)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (4059)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (3944)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3944)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PmeI  (3601)
2 sites
G T T T A A A C C A A A T T T G
PpuMI  (3535)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SanDI  (3535)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
AscI  (3444)
1 site
G G C G C G C C C C G C G C G G
FseI  (3298)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
NaeI  (3296)
1 site
G C C G G C C G G C C