pTK-Cypridina Luc

Control vector for constitutive low-level expression of secreted Cypridina luciferase under control of the HSV TK promoter.

Sequence Author: Thermo Fisher (Pierce)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Luciferase Vectors | More Plasmid Sets
No matches
SpeI (1) PaqCI (6700) DrdI (5720) MauBI (5124) NruI (5051) PvuI (4497) FspI (4349) AfeI (4177) SalI (4017) DraIII (3722) BsmBI - Esp3I (3543) BstEII (3285) PaeR7I - XhoI (7) HindIII (20) BspQI - SapI (155) BsaI (404) BstBI (671) MluI (731) BamHI (778) Bpu10I (952) BstXI (1143) BspDI - ClaI (1214) BsrDI (1271) NheI (1678) BmtI (1682) AleI (1738) BlpI (1744) Bsu36I (2051) KflI (2106) NotI (2453) BseRI (3018) PflFI - Tth111I (3193) BsiWI (3207) RsrII (3267) pTK-Cypridina Luc 6730 bp
SpeI  (1)
1 site
A C T A G T T G A T C A
PaqCI  (6700)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
DrdI  (5720)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
MauBI  (5124)
1 site
C G C G C G C G G C G C G C G C
NruI  (5051)
1 site
T C G C G A A G C G C T
PvuI  (4497)
1 site
C G A T C G G C T A G C
FspI  (4349)
1 site
T G C G C A A C G C G T
AfeI  (4177)
1 site
A G C G C T T C G C G A
SalI  (4017)
1 site
G T C G A C C A G C T G
DraIII  (3722)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsmBI  (3543)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (3543)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
BstEII  (3285)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PaeR7I  (7)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (7)
1 site
C T C G A G G A G C T C
HindIII  (20)
1 site
A A G C T T T T C G A A
BspQI  (155)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (155)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BsaI  (404)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BstBI  (671)
1 site
T T C G A A A A G C T T
MluI  (731)
1 site
A C G C G T T G C G C A
BamHI  (778)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Bpu10I  (952)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BstXI  (1143)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BspDI  (1214)
1 site
A T C G A T T A G C T A
ClaI  (1214)
1 site
A T C G A T T A G C T A
BsrDI  (1271)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
NheI  (1678)
1 site
G C T A G C C G A T C G
BmtI  (1682)
1 site
G C T A G C C G A T C G
AleI  (1738)
1 site
C A C N N N N G T G G T G N N N N C A C
BlpI  (1744)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
Bsu36I  (2051)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
KflI  (2106)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
NotI  (2453)
1 site
G C G G C C G C C G C C G G C G
BseRI  (3018)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
PflFI  (3193)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3193)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsiWI  (3207)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
RsrII  (3267)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
hCluc
790 .. 2451  =  1662 bp
553 amino acids  =  61.5 kDa
Product: secreted Cypridina luciferase
human codon-optimized
hCluc
790 .. 2451  =  1662 bp
553 amino acids  =  61.5 kDa
Product: secreted Cypridina luciferase
human codon-optimized
AmpR
4054 .. 4914  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4054 .. 4845  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4054 .. 4914  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4846 .. 4914  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4054 .. 4914  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
HSV TK promoter
26 .. 777  =  752 bp
herpes simplex virus thymidine kinase promoter
HSV TK promoter
26 .. 777  =  752 bp
herpes simplex virus thymidine kinase promoter
PuroR
3151 .. 3750  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
3151 .. 3750  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
ori
5178 .. 5766  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5178 .. 5766  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
SV40 promoter
2708 .. 3037  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
2708 .. 3037  =  330 bp
SV40 enhancer and early promoter
SV40 poly(A) signal
3880 .. 4001  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3880 .. 4001  =  122 bp
SV40 polyadenylation signal
bGH poly(A) signal
2474 .. 2585  =  112 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
2474 .. 2585  =  112 bp
bovine growth hormone polyadenylation signal
pause site
6639 .. 6730  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
pause site
6639 .. 6730  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
EM7 promoter
3085 .. 3132  =  48 bp
synthetic bacterial promoter
EM7 promoter
3085 .. 3132  =  48 bp
synthetic bacterial promoter
lac operator
6508 .. 6524  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
6508 .. 6524  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
SV40 ori
2888 .. 3023  =  136 bp
SV40 origin of replication
SV40 ori
2888 .. 3023  =  136 bp
SV40 origin of replication
ORF:  790 .. 2451  =  1662 bp
ORF:  553 amino acids  =  61.5 kDa
ORF:  3151 .. 3750  =  600 bp
ORF:  199 amino acids  =  21.5 kDa
ORF:  350 .. 793  =  444 bp
ORF:  147 amino acids  =  16.5 kDa
ORF:  4184 .. 4450  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  912 .. 1529  =  618 bp
ORF:  205 amino acids  =  24.3 kDa
ORF:  1710 .. 2054  =  345 bp
ORF:  114 amino acids  =  13.1 kDa
ORF:  3311 .. 3637  =  327 bp
ORF:  108 amino acids  =  12.0 kDa
ORF:  112 .. 558  =  447 bp
ORF:  148 amino acids  =  16.0 kDa
ORF:  4054 .. 4914  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download pTK-Cypridina Luc.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.