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Plasmid Files

pInvRecA

Plasmid for transiently converting any E. coli strain to a RecA+ phenotype for phage P1-mediated transduction.

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pInvRecA.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Lucigen
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BaeGI - Bme1580I * (7102) ApaLI (7098) ScaI (6911) PvuI (6801) FspI (6653) NmeAIII (6579) BglI (6551) BsaI (6492) AhdI (6431) PluTI (5991) SfoI (5989) NarI (5988) KasI (5987) AflII (5824) AflIII - PciI (5066) BstXI (4416) AatII (4289) ZraI (4287) HpaI (12) BsgI (131) AleI (153) AfeI (287) EcoNI (482) Bpu10I (527) Bsu36I (814) SwaI (1388) MscI * (1794) BmgBI (2134) NheI (2198) BmtI (2202) AvaI - BsoBI - PaeR7I - XhoI (2222) BmeT110I (2223) BlpI (2384) PmeI (2604) BsmBI - PshAI (2695) DrdI (2950) SgrAI (3004) PstI (3140) RsrII (3170) BglII (3177) BamHI (3412) pInvRecA 7354 bp
BaeGI  (7102)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (7102)
1 site
G K G C M C C M C G K G
* Blocked by EcoKI methylation.
Sticky ends from different Bme1580I sites may not be compatible.
ApaLI  (7098)
1 site
G T G C A C C A C G T G
ScaI  (6911)
1 site
A G T A C T T C A T G A
PvuI  (6801)
1 site
C G A T C G G C T A G C
FspI  (6653)
1 site
T G C G C A A C G C G T
NmeAIII  (6579)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (6551)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsaI  (6492)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (6431)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PluTI  (5991)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (5989)
1 site
G G C G C C C C G C G G
NarI  (5988)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (5987)
1 site
G G C G C C C C G C G G
AflII  (5824)
1 site
C T T A A G G A A T T C
AflIII  (5066)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (5066)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstXI  (4416)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
AatII  (4289)
1 site
G A C G T C C T G C A G
ZraI  (4287)
1 site
G A C G T C C T G C A G
HpaI  (12)
1 site
G T T A A C C A A T T G
BsgI  (131)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AleI  (153)
1 site
C A C N N N N G T G G T G N N N N C A C
AfeI  (287)
1 site
A G C G C T T C G C G A
EcoNI  (482)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
Bpu10I  (527)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.