Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT.
NaeI (4136) 1 site
GCCGGCCGGCCG
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV (4134) 1 site
GCCGGCCGGCCG
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BssHII (4031) 1 site
GCGCGCCGCGCG
BssHII is typically used at 50°C, but is 75% active at 37°C.
BanII (3999) 1 site
GRGCYCCYCGRG
Sticky ends from different BanII sites may not be compatible.
PflFI (3752) 1 site
GACNNNGTCCTGNNNCAG
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible.
Tth111I (3752) 1 site
GACNNNGTCCTGNNNCAG
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible.
MscI (3716) 1 site
TGGCCAACCGGT
EagI (3540) 1 site
CGGCCGGCCGGC
PpuMI (3491) 1 site
RGGWCCYYCCWGGR
Sticky ends from different PpuMI sites may not be compatible.
BmeT110I (3430) 1 site
CYCGRGGRGCYC
AbsI (3429) 1 site
CCTCGAGGGGAGCTCC
AvaI (3429) 1 site
CYCGRGGRGCYC
Sticky ends from different AvaI sites may not be compatible.
BsoBI (3429) 1 site
CYCGRGGRGCYC
Sticky ends from different BsoBI sites may not be compatible.BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I (3429) 1 site
CTCGAGGAGCTC
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI (3429) 1 site
VCTCGAGBBGAGCTCV
XhoI (3429) 1 site
CTCGAGGAGCTC
AvrII (3407) 1 site
CCTAGGGGATCC
StuI (3406) 1 site
AGGCCTTCCGGA
SfiI (3360) 1 site
GGCCNNNNNGGCCCCGGNNNNNCCGG
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
SexAI (3174) 1 site
ACCWGGTTGGWCCA
* Blocked by Dcm methylation.Sticky ends from different SexAI sites may not be compatible.
AflIII (3078) 1 site
ACRYGTTGYRCA
Sticky ends from different AflIII sites may not be compatible.
PciI (3078) 1 site
ACATGTTGTACA
PciI is inhibited by nonionic detergents.
AlwNI (2669) 1 site
CAGNNNCTGGTCNNNGAC
Sticky ends from different AlwNI sites may not be compatible.
AhdI (2190) 1 site
GACNNNNNGTCCTGNNNNNCAG
The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible.
EcoRV (4550) 2 sites
GATATCCTATAG
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
EcoRI (1) 1 site
GAATTCCTTAAG
SnaBI (339) 1 site
TACGTAATGCAT
BamHI (581) 1 site
GGATCCCCTAGG
After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI (587) 1 site
TCTAGAAGATCT
SalI (593) 1 site
GTCGACCAGCTG
AccI (594) 1 site
GTMKACCAKMTG
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible.
SbfI (603) 1 site
CCTGCAGGGGACGTCC
HindIII (611) 1 site
AAGCTTTTCGAA
PasI (741) 1 site
CCCWGGGGGGWCCC
Sticky ends from different PasI sites may not be compatible.
EcoRV (793) 2 sites
GATATCCTATAG
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
ClaI (795) 2 sites
ATCGATTAGCTA
SspI (1385) 1 site
AATATTTTATAA
XmnI (1590) 1 site
GAANNNNTTCCTTNNNNAAG
ScaI (1709) 1 site
AGTACTTCATGA
AseI (2015) 1 site
ATTAATTAATTA
BpmI (2121) 1 site
CTGGAG(N)14NNGACCTC(N)14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.BpmI quickly loses activity at 37°C.
AmpR 1403 .. 2263 = 861 bp 286 amino acids = 31.6 kDa Segment 1: signal sequence 1403 .. 1471 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR 1403 .. 2263 = 861 bp 286 amino acids = 31.6 kDa Segment 2: 1472 .. 2263 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR 1403 .. 2263 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR 3506 .. 4300 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR 3506 .. 4300 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori 2434 .. 3022 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori 2434 .. 3022 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
SV40 promoter 3093 .. 3422 = 330 bp SV40 enhancer and early promoter
SV40 promoter 3093 .. 3422 = 330 bp SV40 enhancer and early promoter
CMV enhancer 60 .. 363 = 304 bp human cytomegalovirus immediate early enhancer
CMV enhancer 60 .. 363 = 304 bp human cytomegalovirus immediate early enhancer
CMV promoter 364 .. 567 = 204 bp human cytomegalovirus (CMV) immediate early promoter
CMV promoter 364 .. 567 = 204 bp human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal 4302 .. 4436 = 135 bp SV40 polyadenylation signal
SV40 poly(A) signal 4302 .. 4436 = 135 bp SV40 polyadenylation signal
AmpR promoter 1298 .. 1402 = 105 bp
AmpR promoter 1298 .. 1402 = 105 bp
HSV TK poly(A) signal 674 .. 721 = 48 bp herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
HSV TK poly(A) signal 674 .. 721 = 48 bp herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
MCS 581 .. 616 = 36 bp multiple cloning site
MCS 581 .. 616 = 36 bp multiple cloning site
M13 fwd 808 .. 824 = 17 bp common sequencing primer, one of multiple similar variants
M13 fwd 808 .. 824 = 17 bp common sequencing primer, one of multiple similar variants
SV40 ori 3273 .. 3408 = 136 bp SV40 origin of replication
SV40 ori 3273 .. 3408 = 136 bp SV40 origin of replication