pCEP4

Episomal mammalian vector with a CMV promoter and a hygromycin resistance marker, for high-level expression in primate and canine cells.

Sequence Author: Thermo Fisher (Invitrogen)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
No matches
BsrGI (10,148) AsiSI (8773) PshAI (8436) BstBI (8260) NruI (7959) XmnI (6358) BstEII (5867) EcoNI (5862) StuI * (5791) AvrII (5502) SnaBI (359) Acc65I (620) KpnI (624) PvuII (627) HindIII (636) NotI (647) PaeR7I - PspXI - XhoI (653) NgoMIV (659) NaeI (661) SfiI (665) BamHI (671) EcoRV (1661) NsiI (3212) CsiI - SexAI * (3386) BbvCI (3827) SgrAI (4028) pCEP4 10,186 bp
BsrGI  (10,148)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
AsiSI  (8773)
1 site
G C G A T C G C C G C T A G C G
PshAI  (8436)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BstBI  (8260)
1 site
T T C G A A A A G C T T
NruI  (7959)
1 site
T C G C G A A G C G C T
XmnI  (6358)
1 site
G A A N N N N T T C C T T N N N N A A G
BstEII  (5867)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
EcoNI  (5862)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
StuI  (5791)
1 site
A G G C C T T C C G G A
* Blocked by Dcm methylation.
AvrII  (5502)
1 site
C C T A G G G G A T C C
SnaBI  (359)
1 site
T A C G T A A T G C A T
Acc65I  (620)
1 site
G G T A C C C C A T G G
KpnI  (624)
1 site
G G T A C C C C A T G G
PvuII  (627)
1 site
C A G C T G G T C G A C
HindIII  (636)
1 site
A A G C T T T T C G A A
NotI  (647)
1 site
G C G G C C G C C G C C G G C G
PaeR7I  (653)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (653)
1 site
V C T C G A G B B G A G C T C V
XhoI  (653)
1 site
C T C G A G G A G C T C
NgoMIV  (659)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (661)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
SfiI  (665)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BamHI  (671)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRV  (1661)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NsiI  (3212)
1 site
A T G C A T T A C G T A
CsiI  (3386)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (3386)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BbvCI  (3827)
1 site
C C T C A G C G G A G T C G
SgrAI  (4028)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
EBNA1
3620 .. 5545  =  1926 bp
641 amino acids  =  56.4 kDa
Product: Epstein-Barr nuclear antigen 1, also known as EBNA-1
crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)
EBNA1
3620 .. 5545  =  1926 bp
641 amino acids  =  56.4 kDa
Product: Epstein-Barr nuclear antigen 1, also known as EBNA-1
crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)
oriP
1529 .. 3318  =  1790 bp
Epstein-Barr virus oriP replication origin (Yates et al., 2000)
oriP
1529 .. 3318  =  1790 bp
Epstein-Barr virus oriP replication origin (Yates et al., 2000)
HygR
8409 .. 9446  =  1038 bp
345 amino acids  =  38.5 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
HygR
8409 .. 9446  =  1038 bp
345 amino acids  =  38.5 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
AmpR
6171 .. 7031  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   6171 .. 6239  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6171 .. 7031  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   6240 .. 7031  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6171 .. 7031  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
7202 .. 7790  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7202 .. 7790  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
4 .. 383  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
4 .. 383  =  380 bp
human cytomegalovirus immediate early enhancer
CMV promoter
384 .. 587  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
384 .. 587  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
HSV TK promoter
8221 .. 8366  =  146 bp
herpes simplex virus thymidine kinase promoter
HSV TK promoter
8221 .. 8366  =  146 bp
herpes simplex virus thymidine kinase promoter
SV40 poly(A) signal
672 .. 806  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
672 .. 806  =  135 bp
SV40 polyadenylation signal
AmpR promoter
6066 .. 6170  =  105 bp
AmpR promoter
6066 .. 6170  =  105 bp
HSV TK poly(A) signal
9488 .. 9536  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
HSV TK poly(A) signal
9488 .. 9536  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
MCS
620 .. 676  =  57 bp
multiple cloning site
MCS
620 .. 676  =  57 bp
multiple cloning site
ORF:  4333 .. 5547  =  1215 bp
ORF:  404 amino acids  =  45.2 kDa
ORF:  9736 .. 10,029  =  294 bp
ORF:  97 amino acids  =  11.1 kDa
ORF:  1061 .. 1471  =  411 bp
ORF:  136 amino acids  =  14.7 kDa
ORF:  4142 .. 4378  =  237 bp
ORF:  78 amino acids  =  8.9 kDa
ORF:  7760 .. 8092  =  333 bp
ORF:  110 amino acids  =  12.3 kDa
ORF:  9743 .. 10,051  =  309 bp
ORF:  102 amino acids  =  11.7 kDa
ORF:  6171 .. 7031  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  8409 .. 9446  =  1038 bp
ORF:  345 amino acids  =  38.5 kDa
ORF:  3620 .. 5545  =  1926 bp
ORF:  641 amino acids  =  56.4 kDa
ORF:  6635 .. 6901  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  7709 .. 7945  =  237 bp
ORF:  78 amino acids  =  8.9 kDa
ORF:  1021 .. 1254  =  234 bp
ORF:  77 amino acids  =  8.3 kDa
ORF:  3220 .. 3537  =  318 bp
ORF:  105 amino acids  =  11.7 kDa
ORF:  4318 .. 5430  =  1113 bp
ORF:  370 amino acids  =  40.7 kDa
ORF:  7465 .. 7863  =  399 bp
ORF:  132 amino acids  =  14.8 kDa
ORF:  7927 .. 8205  =  279 bp
ORF:  92 amino acids  =  10.5 kDa
ORF:  2241 .. 2480  =  240 bp
ORF:  79 amino acids  =  8.7 kDa
ORF:  8070 .. 9737  =  1668 bp
ORF:  555 amino acids  =  60.8 kDa
Click here to try SnapGene

Download pCEP4.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.