Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C.
PfoI (3038) 1 site
TCCNGGAAGGNCCT
Sticky ends from different PfoI sites may not be compatible.
BstBI (2945) 1 site
TTCGAAAAGCTT
RsrII (2779) 1 site
CGGWCCGGCCWGGC
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT.
BsrDI (2496) 1 site
GCAATGNNCGTTAC
Sticky ends from different BsrDI sites may not be compatible.
PflFI (2381) 1 site
GACNNNGTCCTGNNNCAG
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible.
Tth111I (2381) 1 site
GACNNNGTCCTGNNNCAG
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible.
FspI (2365) 1 site
TGCGCAACGCGT
MscI (2345) 1 site
TGGCCAACCGGT
PluTI (2266) 1 site
GGCGCCCCGCGG
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI (2264) 1 site
GGCGCCCCGCGG
NarI (2263) 1 site
GGCGCCCCGCGG
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI (2262) 1 site
GGCGCCCCGCGG
BsaBI (2122) 1 site
GATNNNNATCCTANNNNTAG
* Blocked by Dam methylation.
NdeI (240) 1 site
CATATGGTATAC
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI (346) 1 site
TACGTAATGCAT
NheI (597) 1 site
GCTAGCCGATCG
BmtI (601) 1 site
GCTAGCCGATCG
Eco53kI (653) 1 site
GAGCTCCTCGAG
SacI (655) 1 site
GAGCTCCTCGAG
AleI (661) 1 site
CACNNNNGTGGTGNNNNCAC
SacII (662) 1 site
CCGCGGGGCGCC
Efficient cleavage requires at least two copies of the SacII recognition sequence.
BstXI (663) 1 site
CCANNNNNNTGGGGTNNNNNNACC
Sticky ends from different BstXI sites may not be compatible.
NotI (668) 1 site
GCGGCCGCCGCCGGCG
SrfI (771) 1 site
GCCCGGGCCGGGCCCG
BamHI (777) 1 site
GGATCCCCTAGG
After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
PstI (793) 1 site
CTGCAGGACGTC
EcoRI (795) 1 site
GAATTCCTTAAG
EcoRV (803) 1 site
GATATCCTATAG
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
HindIII (807) 1 site
AAGCTTTTCGAA
SalI (822) 1 site
GTCGACCAGCTG
AccI (823) 1 site
GTMKACCAKMTG
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible.