Resources
Plasmid Files

pCMV-DDK-C

Mammalian vector for CMV promoter-driven expression of an ORF with a C-terminal FLAG® tag.

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pCMV-DDK-C Sequence and MappCMV-DDK-C.dna
Map and Sequence File   
Sequence Author:  transOMIC
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 EcoRI (832) SalI (839) AccI (840) BglII (847) AvaI - BsoBI - PaeR7I - XhoI (853) BmeT110I (854) Acc65I (858) KpnI (862) EagI - NotI (895) AanI (1019) pCMV-DDK-C 3766 bp
EcoRI  (832)
1 site
G A A T T C C T T A A G
SalI  (839)
1 site
G T C G A C C A G C T G
AccI  (840)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BglII  (847)
1 site
A G A T C T T C T A G A
AvaI  (853)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (853)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
PaeR7I  (853)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (853)
1 site
C T C G A G G A G C T C
BmeT110I  (854)
1 site
C Y C G R G G R G C Y C
Acc65I  (858)
1 site
G G T A C C C C A T G G
KpnI  (862)
1 site
G G T A C C C C A T G G
EagI  (895)
1 site
C G G C C G G C C G G C
NotI  (895)
1 site
G C G G C C G C C G C C G G C G
AanI  (1019)
1 site
T T A T A A A A T A T T
AmpR
2311 .. 3171  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   2311 .. 3102  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
2311 .. 3171  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   3103 .. 3171  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
2311 .. 3171  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
1552 .. 2140  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin
of replication
ori
1552 .. 2140  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin
of replication
CMV enhancer
27 .. 330  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
27 .. 330  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
331 .. 534  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
331 .. 534  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 poly(A) signal
905 .. 1039  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
905 .. 1039  =  135 bp
SV40 polyadenylation signal
AmpR promoter
3172 .. 3276  =  105 bp
AmpR promoter
3172 .. 3276  =  105 bp
SV40 intron
672 .. 768  =  97 bp
modified SV40 intron with splice donor and acceptor
sites
SV40 intron
672 .. 768  =  97 bp
modified SV40 intron with splice donor and acceptor
sites
MCS
832 .. 863  =  32 bp
multiple cloning site
MCS
832 .. 863  =  32 bp
multiple cloning site
FLAG
867 .. 890  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an
enterokinase cleavage site
FLAG
867 .. 890  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an
enterokinase cleavage site
M13 rev
1150 .. 1166  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
1150 .. 1166  =  17 bp
common sequencing primer, one of multiple similar
variants
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