Resources
Plasmid Files

pCMV-DDK-C

Mammalian vector for CMV promoter-driven expression of an ORF with a C-terminal FLAG® tag.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pCMV-DDK-C.dna
Map and Sequence File:    Download    Open   
Sequence Author:  transOMIC
Download Free Trial Get SnapGene Viewer


EcoRI (832) SalI (839) AccI (840) BglII (847) AvaI - BsoBI - PaeR7I - XhoI (853) BmeT110I (854) Acc65I (858) KpnI (862) EagI - NotI (895) AanI (1019) pCMV-DDK-C 3766 bp
EcoRI  (832)
1 site
G A A T T C C T T A A G
SalI  (839)
1 site
G T C G A C C A G C T G
AccI  (840)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BglII  (847)
1 site
A G A T C T T C T A G A
AvaI  (853)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (853)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (853)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (853)
1 site
C T C G A G G A G C T C
BmeT110I  (854)
1 site
C Y C G R G G R G C Y C
Acc65I  (858)
1 site
G G T A C C C C A T G G
KpnI  (862)
1 site
G G T A C C C C A T G G
EagI  (895)
1 site
C G G C C G G C C G G C
NotI  (895)
1 site
G C G G C C G C C G C C G G C G
AanI  (1019)
1 site
T T A T A A A A T A T T
AmpR
2311 .. 3171  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   2311 .. 3102  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2311 .. 3171  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   3103 .. 3171  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2311 .. 3171  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1552 .. 2140  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
ori
1552 .. 2140  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
27 .. 330  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
27 .. 330  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
331 .. 534  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
331 .. 534  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
905 .. 1039  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
905 .. 1039  =  135 bp
SV40 polyadenylation signal
AmpR promoter
3172 .. 3276  =  105 bp
AmpR promoter
3172 .. 3276  =  105 bp
SV40 intron
672 .. 768  =  97 bp
modified SV40 intron with splice donor and acceptor sites
SV40 intron
672 .. 768  =  97 bp
modified SV40 intron with splice donor and acceptor sites
MCS
832 .. 863  =  32 bp
multiple cloning site
MCS
832 .. 863  =  32 bp
multiple cloning site
FLAG
867 .. 890  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
867 .. 890  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
M13 rev
1150 .. 1166  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
1150 .. 1166  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  2441 .. 2707  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3557 .. 36  =  246 bp
ORF:  81 amino acids  =  9.5 kDa
ORF:  784 .. 1056  =  273 bp
ORF:  90 amino acids  =  10.2 kDa
ORF:  2311 .. 3171  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3517 .. 113  =  363 bp
ORF:  120 amino acids  =  13.4 kDa
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2018 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter