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Plasmid Files

pCMV6-A-BSD

PrecisionShuttle™ mammalian vector with a blasticidin resistance marker, for expressing an untagged ORF.

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pCMV6-A-BSD.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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DraIII (5308) XmnI (4755) ScaI (4636) AlwNI (3679) PciI (3263) BspQI - SapI (3147) BstZ17I (2884) BsmI (2832) AanI (2771) AanI (5433) BsrGI (301) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) AsiSI - SgfI (1024) MreI - SgrAI (1026) AscI - BssHII (1030) HindIII (1044) RsrII (1061) MluI (1067) NotI (1077) PaeR7I - PspXI - XhoI (1085) PmeI (1094) FseI (1104) SacII (1108) AleI (1194) XL39 (reverse primer) (1200 .. 1219) BbvCI - Bpu10I (1420) BlpI (1464) BsmBI (1484) BstXI (1571) AgeI (1682) PflMI (1685) XcmI (1777) SexAI * (1869) SfiI (2055) StuI (2101) AvrII (2102) BclI * (2153) BsiWI (2166) PpuMI (2334) NruI * (2406) BsaBI * (2473) PstI (2578) BstBI (2594) BfuAI - BspMI (2630) PfoI (2687) pCMV6-A-BSD 5505 bp
DraIII  (5308)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
XmnI  (4755)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (4636)
1 site
A G T A C T T C A T G A
AlwNI  (3679)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (3263)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (3147)
1 site
G C T C T T C N C G A G A A G N N N N