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pCMV6-A-Hygro

PrecisionShuttle™ mammalian vector with a hygromycin resistance marker, for expressing an untagged ORF.

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pCMV6-A-Hygro.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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AanI (6060) XmnI (5382) AlwNI (4306) PciI (3890) BspQI - SapI (3774) BstZ17I (3511) BsmI (3459) AanI (3398) PfoI (3314) BstBI (3221) BsrGI (301) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) AsiSI - SgfI (1024) MreI - SgrAI (1026) AscI - BssHII (1030) HindIII (1044) MluI (1067) NotI (1077) PaeR7I - PspXI - XhoI (1085) PmeI (1094) FseI (1104) AleI (1194) BbsI (1317) EcoO109I (1324) BsgI (1378) BbvCI - Bpu10I (1420) BlpI (1464) BstXI (1571) PflMI (1685) XcmI (1777) SexAI * (1869) SfiI (2055) StuI (2101) AvrII (2102) BclI * (2153) BsiWI (2166) PstI (3205) pCMV6-A-Hygro 6132 bp
AanI  (6060)
2 sites
T T A T A A A A T A T T
XmnI  (5382)
1 site
G A A N N N N T T C C T T N N N N A A G
AlwNI  (4306)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (3890)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (3774)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3774)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstZ17I  (3511)
1 site
G T A T A C C A T A T G
BsmI  (3459)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
AanI  (3398)
2 sites
T T A T A A A A T A T T
PfoI  (3314)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BstBI  (3221)
1 site
T T C G A A A A G C T T
BsrGI  (301)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SnaBI  (698)
1 site
T A C G T A A T G C A T
Eco53kI  (924)
1 site
G A G C T C C T C G A G
SacI  (926)
1 site
G A G C T C C T C G A G
EcoRI  (979)
1 site
G A A T T C C T T A A G
BamHI  (992)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Acc65I  (998)
1 site
G G T A C C C C A T G G
KpnI  (1002)
1 site
G G T A C C C C A T G G
AsiSI  (1024)
1 site
G C G A T C G C C G C T A G C G
SgfI  (1024)
1 site
G C G A T C G C C G C T A G C G
MreI  (1026)
1 site
C G C C G G C G G C G G C C G C
SgrAI  (1026)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AscI  (1030)
1 site
G G C G C G C C C C G C G C G G
BssHII  (1030)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
HindIII  (1044)
1 site
A A G C T T T T C G A A
MluI  (1067)
1 site
A C G C G T T G C G C A
NotI  (1077)
1 site
G C G G C C G C C G C C G G C G
PaeR7I  (1085)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1085)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1085)
1 site
C T C G A G G A G C T C
PmeI  (1094)
1 site
G T T T A A A C C A A A T T T G
FseI  (1104)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
AleI  (1194)
1 site
C A C N