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pCMV6-A-Puro

PrecisionShuttle™ mammalian vector with a puromycin resistance marker, for expressing an untagged ORF.

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pCMV6-A-Puro.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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AanI (5634) XmnI (4956) ScaI (4837) AlwNI (3880) PciI (3464) BspQI - SapI (3348) BstZ17I (3085) BsmI (3033) AanI (2972) BsrGI (301) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) AsiSI - SgfI (1024) MreI - SgrAI (1026) AscI (1030) HindIII (1044) RsrII (1061) MluI (1067) NotI (1077) PaeR7I - PspXI - XhoI (1085) PmeI (1094) FseI (1104) AleI (1194) XL39 (reverse primer) (1200 .. 1219) BbsI (1317) EcoO109I (1324) BsgI (1378) BbvCI - Bpu10I (1420) BlpI (1464) BstXI (1571) AgeI (1682) PflMI (1685) XcmI (1777) SfiI (2055) AvrII (2102) BclI * (2153) BsiWI (2166) PflFI - Tth111I (2217) BstEII (2309) PstI (2779) BstBI (2795) BfuAI - BspMI (2831) pCMV6-A-Puro 5706 bp
AanI  (5634)
2 sites
T T A T A A A A T A T T
XmnI  (4956)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (4837)
1 site
A G T A C T T C A T G A
AlwNI  (3880)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (3464)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (3348)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3348)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstZ17I  (3085)
1 site
G T A T A C C A T A T G
BsmI  (3033)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
AanI  (2972)
2 sites
T T A T A A A A T A T T
BsrGI  (301)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
NdeI  (592)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (698)
1 site
T A C G T A A T G C A T
Eco53kI  (924)
1 site
G A G C T C C T C G A G
SacI  (926)
1 site
G A G C T C C T C G A G
EcoRI  (979)
1 site
G A A T T C C T T A A G
BamHI  (992)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Acc65I  (998)
1 site
G G T A C C C C A T G G
KpnI  (1002)
1 site
G G T A C C C C