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Plasmid Files

pCMV6-AC-3HA

PrecisionShuttle™ mammalian expression vector for fusing an ORF to a C-terminal 3xHA tag.

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pCMV6-AC-3HA.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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DraIII (5792) XmnI (5239) ScaI (5120) AlwNI (4166) PciI (3750) BstZ17I (3371) BsmI (3319) BstBI (3081) BsrGI (301) SpeI (357) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) Acc65I (998) KpnI (1002) AsiSI (1024) MreI - SgrAI (1026) AscI (1030) HindIII (1044) NheI (1055) BmtI (1059) RsrII (1061) MluI (1067) BsiWI (1070) NotI (1076) PaeR7I - PspXI - XhoI (1082) PmeI (1181) FseI (1191) SacII (1195) AleI (1281) XL39 (reverse primer) (1287 .. 1306) BbsI (1404) EcoO109I (1411) BsgI (1465) BbvCI - Bpu10I (1507) BlpI (1551) BsmBI (1571) BstXI (1658) AgeI (1769) PflMI (1772) XcmI (1864) SexAI * (1956) SfiI (2142) StuI (2188) AvrII (2189) BclI * (2240) BsaBI * (2258) KasI (2398) NarI (2399) SfoI (2400) PluTI (2402) PstI (2452) PflFI - Tth111I (2517) pCMV6-AC-3HA 5989 bp
DraIII  (5792)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
XmnI  (5239)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (5120)
1 site
A G T A C T T C A T G A
AlwNI  (4166)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (3750)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (3371)
1 site
G T A T A C C A T A T G
BsmI  (3319)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BstBI  (3081)
1 site
T T C G A A A A G C T T
BsrGI  (301)
1 site
T G T A