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Plasmid Files

pCMV6-AC-HA

PrecisionShuttle™ mammalian expression vector for fusing an ORF to a C-terminal HA tag.

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pCMV6-AC-HA.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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BsrGI (301) AanI (5854) DraIII (5729) XmnI (5176) ScaI (5057) AlwNI (4103) PciI (3687) BstZ17I (3308) BsmI (3256) AanI (3195) PfoI (3111) BstBI (3018) SpeI (357) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) AsiSI - SgfI (1024) MreI - SgrAI (1026) AscI (1030) HindIII (1044) NheI (1055) BmtI (1059) RsrII (1061) MluI (1067) BsiWI (1070) NotI (1076) PaeR7I - PspXI - XhoI (1082) PmeI (1118) FseI (1128) SacII (1132) AleI (1218) XL39 (reverse primer) (1224 .. 1243) BbsI (1341) EcoO109I (1348) BsgI (1402) BbvCI - Bpu10I (1444) BlpI (1488) BsmBI (1508) BstXI (1595) AgeI (1706) PflMI (1709) XcmI (1801) SexAI * (1893) SfiI (2079) StuI (2125) AvrII (2126) BclI * (2177) BsaBI * (2195) KasI (2335) NarI (2336) SfoI (2337) PluTI (2339) PstI (2389) PflFI - Tth111I (2454) pCMV6-AC-HA 5926 bp
BsrGI  (301)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
AanI  (5854)
2 sites
T T A T A A A A T A T T
DraIII  (5729)
1 site
C A C N N N G T<