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Plasmid Files

pCMV6-AC-mCFP

PrecisionShuttle™ mammalian expression vector for fusing an ORF to a C-terminal TagCFP tag.

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pCMV6-AC-mCFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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AanI (6559) ScaI (5762) AlwNI (4805) PciI (4389) BstZ17I (4010) BsmI (3958) AanI (3897) BstBI (3720) PflFI - Tth111I (3156) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) AsiSI - SgfI (1024) MreI - SgrAI (1026) AscI (1030) HindIII (1044) RsrII (1061) MluI (1067) BsiWI (1070) NotI (1076) PaeR7I - XhoI (1082) BmgBI (1150) BstEII (1267) PasI (1648) BspEI (1802) PmeI (1820) FseI (1830) XL39 (reverse primer) (1926 .. 1945) BbsI (2043) EcoO109I (2050) BbvCI (2146) BlpI (2190) BsmBI (2210) BstXI (2297) AgeI (2408) SexAI * (2595) SfiI (2781) StuI (2827) AvrII (2828) BclI * (2879) KasI (3037) NarI (3038) SfoI (3039) PluTI (3041) PstI (3091) pCMV6-AC-mCFP 6631 bp
AanI  (6559)
2 sites
T T A T A A A A T A T T
ScaI  (5762)
1 site
A G T A C T T C A T G A
AlwNI  (4805)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (4389)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (4010)
1 site
G T A T A C C A T A T G
BsmI  (3958)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
AanI  (3897)
2 sites
T T A T A A A A T A T T
BstBI  (3720)
1 site
T T C G A A A A G C T T
PflFI  (3156)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3156)
1 site
G A C N N N G T