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Plasmid Files

pCMV6-AC-mGFP

PrecisionShuttle™ mammalian expression vector for fusing an ORF to a C-terminal TagGFP2 (mTagGFP) tag.

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pCMV6-AC-mGFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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AanI (6559) ScaI (5762) PciI (4389) BstZ17I (4010) BsmI (3958) AanI (3897) BstBI (3720) PflFI - Tth111I (3156) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) AsiSI - SgfI (1024) MreI - SgrAI (1026) AscI (1030) HindIII (1044) RsrII (1061) MluI (1067) BsiWI (1070) NotI (1076) PaeR7I - XhoI (1082) BmgBI (1150) BstEII (1267) PasI (1648) BspEI (1802) PmeI (1820) FseI (1830) XL39 (reverse primer) (1926 .. 1945) BbsI (2043) EcoO109I (2050) BbvCI (2146) BlpI (2190) BsmBI (2210) BstXI (2297) AgeI (2408) SexAI * (2595) SfiI (2781) StuI (2827) AvrII (2828) BclI * (2879) KasI (3037) NarI (3038) SfoI (3039) PluTI (3041) PstI (3091) pCMV6-AC-mGFP 6631 bp
AanI  (6559)
2 sites
T T A T A A A A T A T T
ScaI  (5762)
1 site
A G T A C T T C A T G A
PciI  (4389)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (4010)
1 site
G T A T A C C A T A T G
BsmI  (3958)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
AanI  (3897)
2 sites
T T A T A A A A T A T T
BstBI  (3720)
1 site
T T C G A A A A G C T T
PflFI  (3156)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3156)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
NdeI  (592)
1