Resources
Plasmid Files

pCMV6-AC-mYFP

PrecisionShuttle™ mammalian expression vector for fusing an ORF to a C-terminal TagYFP tag.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pCMV6-AC-mYFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
Download Free Trial Get SnapGene Viewer


AanI (6562) ScaI (5765) PciI (4392) BstZ17I (4013) BsmI (3961) AanI (3900) BstBI (3723) PflFI - Tth111I (3159) BsrGI (301) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) AsiSI - SgfI (1024) MreI - SgrAI (1026) AscI (1030) HindIII (1044) RsrII (1061) MluI (1067) BsiWI (1070) NotI (1076) PaeR7I - XhoI (1082) BmgBI (1153) BstEII (1270) PasI (1651) BspEI (1805) PmeI (1823) FseI (1833) XL39 (reverse primer) (1929 .. 1948) BbsI (2046) EcoO109I (2053) BbvCI (2149) BlpI (2193) BsmBI (2213) BstXI (2300) AgeI (2411) SexAI * (2598) SfiI (2784) StuI (2830) AvrII (2831) BclI * (2882) KasI (3040) NarI (3041) SfoI (3042) PluTI (3044) pCMV6-AC-mYFP 6634 bp
AanI  (6562)
2 sites
T T A T A A A A T A T T
ScaI  (5765)
1 site
A G T A C T T C A T G A
PciI  (4392)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (4013)
1 site
G T A T A C C A T A T G
BsmI  (3961)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
AanI  (3900)
2 sites
T T A T A A A A T A T T
BstBI  (3723)
1 site
T T C G A A A A G C T T
PflFI  (3159)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3159)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsrGI  (301)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
NdeI  (592)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (698)
1 site
T A C G T A A T G C A T
Eco53kI  (924)
1 site
G A G C T C C T C G A G
SacI  (926)
1 site
G A G C T C C T C G A G
EcoRI  (979)
1 site
G A A T T C C T T A A G
BamHI  (992)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Acc65I  (998)
1 site
G G T A C C C C A T G G
KpnI  (1002)
1 site
G G T A C C C C A T G G
AsiSI  (1024)
1 site
G C G A T C G C C G C T A G C G
SgfI  (1024)
1 site
G C G A T C G C C G C