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Plasmid Files

pCMV6-AC

PrecisionShuttle™ mammalian vector with a neomycin resistance marker, for expressing an untagged ORF.

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pCMV6-AC.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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AanI (5830) DraIII (5705) XmnI (5152) ScaI (5033) AlwNI (4079) PciI (3663) BstZ17I (3284) BsmI (3232) AanI (3171) PfoI (3087) BstBI (2994) BsrGI (301) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) AsiSI - SgfI (1024) MreI - SgrAI (1026) AscI (1030) HindIII (1044) RsrII (1061) MluI (1067) NotI (1077) PaeR7I - PspXI - XhoI (1085) PmeI (1094) FseI (1104) SacII (1108) AleI (1194) XL39 (reverse primer) (1200 .. 1219) BbsI (1317) EcoO109I (1324) BsgI (1378) BbvCI - Bpu10I (1420) BlpI (1464) BsmBI (1484) BstXI (1571) AgeI (1682) PflMI (1685) XcmI (1777) SexAI * (1869) SfiI (2055) StuI (2101) AvrII (2102) BclI * (2153) BsaBI * (2171) KasI (2311) NarI (2312) SfoI (2313) PluTI (2315) PstI (2365) PflFI - Tth111I (2430) pCMV6-AC 5902 bp
AanI  (5830)
2 sites
T T A T A A A A T A T T
DraIII  (5705)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
XmnI  (5152)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (5033)
1 site
A G T A C T T C A T G A
AlwNI  (4079)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (3663)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (3284)
1 site
G T A T A C C A T A T G
BsmI  (3232)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
AanI  (3171)
2 sites
T T A T A A A A T A T T
PfoI  (3087)
1 site