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Plasmid Files

pCMV6-AN-RFP

PrecisionShuttle™ mammalian expression vector for fusing an ORF to an N-terminal TurboRFP tag.

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pCMV6-AN-RFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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AanI (6523) XmnI (5845) AlwNI (4772) PciI (4356) BstZ17I (3977) BsmI (3925) AanI (3864) PfoI (3780) BstBI (3687) PflFI - Tth111I (3123) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) PasI (1238) Bsu36I (1273) PshAI (1647) AsiSI - SgfI (1717) MreI - SgrAI (1719) AscI (1723) HindIII (1737) RsrII (1754) MluI (1760) NotI (1770) PaeR7I - PspXI - XhoI (1778) PmeI (1787) FseI (1797) SacII (1801) AleI (1887) XL39 (reverse primer) (1893 .. 1912) EcoO109I (2017) BbvCI - Bpu10I (2113) BlpI (2157) BsmBI (2177) BstXI (2264) AgeI (2375) XcmI (2470) SexAI * (2562) StuI (2794) AvrII (2795) BsaBI * (2864) KasI (3004) NarI (3005) SfoI (3006) PluTI (3008) PstI (3058) pCMV6-AN-RFP 6595 bp
AanI  (6523)
2 sites
T T A T A A A A T A T T
XmnI  (5845)
1 site
G A A N N N N T T C C T T N N N N A A G
AlwNI  (4772)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (4356)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (3977)
1 site
G T A T A C C A T A T G
BsmI  (3925)
1 site
G A A T