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pCMV6-Entry-Cre

Mammalian vector for expressing myc- and FLAG®-tagged Cre to enable Cre-Lox recombination.

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pCMV6-Entry-Cre.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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DraIII (5713) SfiI (5054) StuI (5005) PluTI (4827) SfoI (4825) NarI (4824) KasI (4823) PflFI - Tth111I (4707) ApaLI (3218) BsrGI (301) SpeI (357) AseI (365) NdeI (592) Eco53kI (924) SacI (926) EcoRI (979) SalI (985) AccI (986) Acc65I (998) KpnI (1002) BglII (1008) XmnI (1237) NruI (1243) BssHII (1266) RsrII (1330) AclI (1399) AarI (1638) PshAI (1864) MluI (2058) BsiWI (2061) NotI (2067) PaeR7I - PspXI - XhoI (2073) PmeI (2151) FseI (2161) SacII (2165) TspMI - XmaI (2191) SmaI (2193) AleI (2251) AhdI (2307) BbsI (2374) BsgI (2435) BbvCI (2477) BlpI (2521) BstXI (2628) PflMI (2742) pCMV6-Entry-Cre 5910 bp
DraIII  (5713)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SfiI  (5054)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
StuI  (5005)
1 site
A G G C C T T C C G G A
PluTI  (4827)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (4825)
1 site
G G C G C C C C G C G G
NarI  (4824)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (4823)
1 site
G G C G C C C C G C G G
PflFI  (4707)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4707)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
ApaLI  (3218)
1 site
G T G C A C C A C G T G
BsrGI  (301)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SpeI  (357)
1 site
A C T A G T T G A T C A
AseI  (365)
1 site
A T T A A T T A A T T A
NdeI  (592)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
Eco53kI  (924)
1 site
G A G C T C C T C G A G
SacI  (926)
1 site
G A G C T C C T C G A G
EcoRI  (979)
1 site
G A A T T C C T T A A G
SalI  (985)
1 site
G T C G A C C A G C T G
AccI  (986)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (998)
1 site
G G T A C C C C A T G G
KpnI  (1002)
1 site
G G T A C C C C A T G G
BglII  (1008)
1 site
A G A T C T T C T A G A
XmnI  (1237)
1 site
G A A N N N N T T C C T T N N N N A A G
NruI  (1243)
1 site
T C G C G A A G C G C T
BssHII  (1266)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
RsrII  (1330)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AclI  (1399)
1 site
A A C G T T T T G C A A
AarI  (1638)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
PshAI  (1864)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
MluI  (2058)
1 site
A C G C G T T G C G C A
BsiWI  (2061)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
NotI  (2067)
1 site
G C G G C C G C C G C C G G C G
PaeR7I  (2073)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (2073)
1 site
V C T C G A G B B G A G C T C V
XhoI  (2073)
1 site