Resources
Plasmid Files

pCMV6-Entry

Mammalian expression and dual tagging vector that serves as an entry vector to transfer an ORF into a destination vector using the PrecisionShuttle™ system.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pCMV6-Entry.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
Download Free Trial Get SnapGene Viewer


AanI - PsiI (4847) DraIII (4722) SexAI * (4241) SfiI (4063) StuI (4014) BspDI * - ClaI * (3993) PluTI (3836) SfoI (3834) NarI (3833) KasI (3832) PflFI - Tth111I (3716) BstBI (3151) BsrGI (301) SpeI (357) AseI (365) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) SalI (985) AccI (986) BamHI (992) Acc65I (998) KpnI (1002) AsiSI - SgfI (1024) MreI - SgrAI (1026) AscI - BssHII (1030) HindIII (1044) NheI (1055) BmtI (1059) RsrII (1061) MluI (1067) BsiWI (1070) NotI (1076) PaeR7I - PspXI - XhoI (1082) EcoRV (1126) PmeI (1160) FseI (1170) SacII (1174) TspMI - XmaI (1200) SmaI (1202) AleI (1260) XL39 (reverse primer) (1266 .. 1285) AhdI (1316) BbsI (1383) BsgI (1444) BbvCI - Bpu10I (1486) BlpI (1530) BsmBI (1550) BstXI (1637) AgeI (1748) PflMI (1751) PciI (1913) ApaLI (2227) pCMV6-Entry 4919 bp
AanI  (4847)
1 site
T T A T A A A A T A T T
PsiI  (4847)
1 site
T T A T A A A A T A T T
DraIII  (4722)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SexAI  (4241)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
SfiI  (4063)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
StuI  (4014)
1 site
A G G C C T T C C G G A
BspDI  (3993)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (3993)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
PluTI  (3836)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (3834)
1 site
G G C G C C C C G C G G
NarI  (3833)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (3832)
1 site
G G C G C C C C G C G G
PflFI  (3716)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3716)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BstBI  (3151)
1 site
T T C G A A A A G C T T
BsrGI  (301)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SpeI  (357)
1 site
A C T A G T T G A T C A
AseI  (365)
1 site
A T T A A T T A A T T A
NdeI  (592)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (698)
1 site
T A C G T A A T G C A T
Eco53kI  (924)
1 site
G A G C T C C T C G A G
SacI  (926)
1 site
G A G C T C C T C G A G
EcoRI  (979)
1 site
G A A T T C C T T A A G
SalI  (985)
1 site
G T C G A C C A G C T G
AccI  (986)
1 site
G