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Plasmid Files

pCMV6-Entry2

Mammalian expression and dual tagging vector that serves as a secondary entry vector to transfer an ORF into a destination vector using the PrecisionShuttle™ system.

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pCMV6-Entry2.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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BsrGI (301) AanI (5815) DraIII (5690) NruI (5244) EcoNI (4988) AlwNI (4145) ApaLI (4043) PciI (3729) BstZ17I (3350) AanI (3237) BstBI (3060) SpeI (357) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) AsiSI - SgfI (1024) MreI - SgrAI (1026) AscI (1030) HindIII (1044) NheI (1055) BmtI (1059) RsrII (1061) MluI (1067) BsiWI (1070) NotI (1076) PaeR7I - PspXI - XhoI (1082) EcoRV (1126) PmeI (1160) FseI (1170) SacII (1174) AleI (1260) XL39 (reverse primer) (1266 .. 1285) AhdI (1316) BbsI (1383) EcoO109I (1390) BsgI (1444) BbvCI (1486) BlpI (1530) BsaI (1592) BstXI (1637) AgeI (1748) XcmI (1843) SexAI * (1935) SfiI (2121) StuI (2167) AvrII (2168) BclI * (2219) BsaBI * (2237) KasI (2377) NarI (2378) SfoI (2379) PluTI (2381) PstI (2431) PflFI - Tth111I (2496) pCMV6-Entry2 5887 bp
BsrGI  (301)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
AanI  (5815)
2 sites
T T A T A A A A T A T T
DraIII  (5690)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
NruI  (5244)
1 site
T C G C G A A G C G C T
EcoNI  (4988)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
AlwNI  (4145)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
ApaLI  (4043)
1 site
G T G C A C C A C G T G
PciI  (3729)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (3350)
1 site
G T A T A C C A T A T G
AanI  (3237)
2 sites
T T A T A A A A T A T T
BstBI  (3060)
1 site
T T C G A A A A G C T T
SpeI  (357)
1 site
A C T A G T T G A T C A
NdeI  (592)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (698)
1 site
T A C G T A A T G C A T
Eco53kI  (924)
1 site
G A G C T C C T C G A G
SacI  (926)
1 site
G A G C T C C T C G A G
EcoRI  (979)
1 site
G A A T T C C T T A A G
BamHI  (992)
1 site