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Plasmid Files

pDisplay™

Mammalian vector for expressing HA- and myc-tagged proteins that are anchored to the cell surface.

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pDisplay.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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HindIII (664) Acc65I (670) KpnI (674) BstXI (709) EcoRI (713) EcoRV (729) PspOMI (828) ApaI (832) SfiI (836) BglII (842) TspMI - XmaI (848) SmaI (850) SacII (859) PstI (866) SalI (868) AccI (869) NotI (1058) PaeR7I - XhoI (1064) pDisplay™ 5325 bp
HindIII  (664)
1 site
A A G C T T T T C G A A
Acc65I  (670)
1 site
G G T A C C C C A T G G
KpnI  (674)
1 site
G G T A C C C C A T G G
BstXI  (709)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EcoRI  (713)
1 site
G A A T T C C T T A A G
EcoRV  (729)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PspOMI  (828)
1 site
G G G C C C C C C G G G
ApaI  (832)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SfiI  (836)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BglII  (842)
1 site
A G A T C T T C T A G A
TspMI  (848)
1 site
C C C G G G G G G C C C
XmaI  (848)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (850)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
SacII  (859)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PstI  (866)
1 site
C T G C A G G A C G T C
SalI  (868)
1 site
G T C G A C C A G C T G
AccI  (869)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
NotI  (1058)
1 site
G C G G C C G C C G C C G G C G
PaeR7I  (1064)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1064)
1 site
C T C G A G G A G C T C
AmpR
3876 .. 4736  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   3876 .. 4667  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3876 .. 4736  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   4668 .. 4736  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3876 .. 4736  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
2633 .. 3421  =  789 bp
262 amino acids  =  28.8 kDa
Product: aminoglycoside phosphotransferase
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
2633 .. 3421  =  789 bp
262 amino acids  =  28.8 kDa
Product: aminoglycoside phosphotransferase
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
1437 .. 2025  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1437 .. 2025  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
4868 .. 5323  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
4868 .. 5323  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
10 .. 389  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
10 .. 389  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
3456 .. 3816  =  361 bp
SV40 enhancer and early promoter
SV40 promoter
3456 .. 3816  =  361 bp
SV40 enhancer and early promoter
bGH poly(A) signal
1081 .. 1305  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
1081 .. 1305  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
390 .. 593  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
390 .. 593  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
PDGFRβ TM domain
907 .. 1053  =  147 bp
49 amino acids  =  5.4 kDa
Product: transmembrane domain from platelet derived growth factor receptor beta
PDGFRβ TM domain
907 .. 1053  =  147 bp
49 amino acids  =  5.4 kDa
Product: transmembrane domain from platelet derived growth factor receptor beta
AmpR promoter
4737 .. 4841  =  105 bp
AmpR promoter
4737 .. 4841  =  105 bp
Igκ leader
737 .. 799  =  63 bp
21 amino acids  =  2.4 kDa
Product: leader sequence from mouse immunoglobulin κ light chain
Igκ leader
737 .. 799  =  63 bp
21 amino acids  =  2.4 kDa
Product: leader sequence from mouse immunoglobulin κ light chain
HSV TK poly(A) signal
2354 .. 2401  =  48 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
HSV TK poly(A) signal
2354 .. 2401  =  48 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
MCS
828 .. 873  =  46 bp
multiple cloning site
MCS
828 .. 873  =  46 bp
multiple cloning site
Myc
874 .. 903  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc proto-oncogene) epitope tag
Myc
874 .. 903  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc proto-oncogene) epitope tag
HA
800 .. 826  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
HA
800 .. 826  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
T7 promoter
638 .. 656  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
638 .. 656  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
3470 .. 3608  =  139 bp
SV40 origin of replication
SV40 ori
3470 .. 3608  =  139 bp
SV40 origin of replication
ORF:  4006 .. 4272  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2174 .. 2407  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
ORF:  2582 .. 3112  =  531 bp
ORF:  176 amino acids  =  19.6 kDa
ORF:  2869 .. 3249  =  381 bp
ORF:  126 amino acids  =  14.3 kDa
ORF:  600 .. 1031  =  432 bp
ORF:  143 amino acids  =  15.9 kDa
ORF:  1071 .. 1379  =  309 bp
ORF:  102 amino acids  =  11.3 kDa
ORF:  2163 .. 2612  =  450 bp
ORF:  149 amino acids  =  16.3 kDa
ORF:  3876 .. 4736  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  2633 .. 3421  =  789 bp
ORF:  262 amino acids  =  28.8 kDa
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