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Plasmid Files

pEF6/V5-His C

Mammalian vector for high-level constitutive expression of C-terminally V5- and 6xHis-tagged proteins. For other reading frames, use pEF6/V5‑His A or pEF6/V5‑His B.

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pEF6 V5-His C.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SspI (3) Acc113I - BmcAI - ScaI - ZrmI (5482) Acc16I - AviII - FspI - NsbI (5224) AhdI - AspEI - BmeRI - DriI - Eam1105I (5002) BspQI - LguI - PciSI - SapI (4004) BssNAI - Bst1107I - BstZ17I (3741) BsaMI - BsmI - Mva1269I - PctI (3689) PfoI (3544) BlpI - Bpu1102I - Bsp1720I - CelII (3448) BsaBI * - Bse8I - BseJI * - MamI * (3351) PpuMI - Psp5II - PspPPI (3212) BalI - MlsI - MluNI - MscI - Msp20I (3056) SmaI (2947) Cfr9I - TspMI - XmaI - XmaCI (2945) ZraI (119) SgrDI (120) AatII (121) AflII - BfrI - BspTI - Bst98I - BstAFI - MspCI - Vha464I (852) Acc36I - BfuAI - BspMI - BveI (997) Bsp120I - PspOMI (1086) ApaI (1090) FseI - RigI (1177) PasI (1221) BbvCI (1375) KasI - SspDI (1411) Mly113I - NarI (1412) DinI - EgeI - EheI - SfoI (1413) BbeI - PluTI (1415) Acc65I - Asp718I (1713) KpnI (1717) BamHI (1725) AhlI - BcuI - SpeI (1731) EcoRI (1748) Eco32I - EcoRV (1760) CciNI - NotI (1775) PspXI (1781) BstEII - BstPI - Eco91I - EcoO65I - PspEI (1786) AsuII - Bpu14I - Bsp119I - BspT104I - BstBI - Csp45I - NspV - SfuI (1796) MssI - PmeI (1876) CsiI - MabI - SexAI * (2691) AspA2I - AvrII - BlnI - XmaJI (2924)