Resources
Plasmid Files

pFC15A HaloTag® CMVd1

Flexi® vector with an ampicillin resistance marker, for mammalian expression from a slightly compromised CMV promoter of a protein with a C-terminal HaloTag®.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pFC15A HaloTag CMVd1 Sequence and MappFC15A HaloTag CMVd1.dna
Map and Sequence File   
Sequence Author:  Promega
Download Free Trial Get SnapGene Viewer

 BsaBI * (4176) MluI (4058) BspEI (3845) PciI (3081) AgeI (3013) BstBI (2986) AseI (2736) FspI (2688) XmnI (2311) SspI (2106) BglII (4177) HindIII (127) AsiSI - PvuI - SgfI (186) BtgI - NcoI (189) Eco53kI - EcoICRI (570) SacI (572) PaeR7I - XhoI (573) PflMI (659) AleI (717) BstEII (735) BseRI (737) PflFI - Tth111I (867) BmgBI (869) PasI (899) StuI * (1068) BclI * (1099) BspDI * - ClaI * (1105) PshAI (1164) SgrAI (1251) BbsI (1450) BssHII (1476) XbaI (1518) SalI (1524) PstI - SbfI (1534) BfuAI - BspMI (1537) BlpI (1599) NotI (1629) AanI - PsiI (1752) HpaI (1772) MfeI (1781) SnaBI (1973) pFC15A HaloTag® CMVd1 4182 bp
BsaBI  (4176)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
MluI  (4058)
1 site
A C G C G T T G C G C A
BspEI  (3845)
1 site
T C C G G A A G G C C T
PciI  (3081)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AgeI  (3013)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BstBI  (2986)
1 site
T T C G A A A A G C T T
AseI  (2736)
1 site
A T T A A T T A A T T A
FspI  (2688)
1 site
T G C G C A A C G C G T
XmnI  (2311)
1 site
G A A N N N N T T C C T T N N N N A A G
SspI  (2106)
1 site
A A T A T T T T A T A A
BglII  (4177)
1 site
A G A T C T T C T A G A
HindIII  (127)
1 site
A A G C T T T T C G A A
AsiSI  (186)
1 site
G C G A T C G C C G C T A G C G
PvuI  (186)
1 site
C G A T C G G C T A G C
SgfI  (186)
1 site
G C G A T C G C C G C T A G C G
BtgI  (189)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (189)
1 site
C C A T G G G G T A C C
Eco53kI  (570)
1 site
G A G C T C C T C G A G
EcoICRI  (570)
1 site
G A G C T C C T C G A G
SacI  (572)
1 site
G A G C T C C T C G A G
PaeR7I  (573)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (573)
1 site
C T C G A G G A G C T C
PflMI  (659)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AleI  (717)
1 site
C A C N N N N G T G G T G N N N N C A C
BstEII  (735)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BseRI  (737)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
PflFI  (867)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (867)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BmgBI  (869)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
PasI  (899)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
StuI  (1068)
1 site
A G G C C T T C C G G A
* Blocked by Dcm methylation.
BclI  (1099)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BspDI  (1105)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (1105)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
PshAI  (1164)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
SgrAI  (1251)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
BbsI  (1450)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BssHII  (1476)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
XbaI  (1518)
1 site
T C T A G A A G A T C T
SalI  (1524)
1 site
G T C G A C C A G C T G
PstI  (1534)
1 site
C T G C A G G A C G T C
SbfI  (1534)
1 site
C C T G C A G G G G A C G T C C
BfuAI  (1537)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (1537)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BlpI  (1599)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
NotI  (1629)
1 site
G C G G C C G C C G C C G G C G
AanI  (1752)
1 site
T T A T A A A A T A T T
PsiI  (1752)
1 site
T T A T A A A A T A T T
HpaI  (1772)
1 site
G T T A A C C A A T T G
MfeI  (1781)
1 site
C A A T T G G T T A A C
SnaBI  (1973)
1 site
T A C G T A A T G C A T
HaloTag®
624 .. 1505  =  882 bp
294 amino acids  =  33.4 kDa
Product: modified bacterial dehalogenase that forms
covalent bonds with chloroalkane derivatives
HaloTag®
624 .. 1505  =  882 bp
294 amino acids  =  33.4 kDa
Product: modified bacterial dehalogenase that forms
covalent bonds with chloroalkane derivatives
AmpR
2124 .. 2984  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   2124 .. 2192  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
2124 .. 2984  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   2193 .. 2984  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
2124 .. 2984  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
3142 .. 3730  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3142 .. 3730  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
barnase
213 .. 548  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus
amyloliquefaciens

The barnase gene is lethal in standard bacterial
transformation strains.
barnase
213 .. 548  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus
amyloliquefaciens

The barnase gene is lethal in standard bacterial
transformation strains.
cer region
3846 .. 4129  =  284 bp
ColE1-derived recombination site that helps to
maintain plasmids as monomers
cer region
3846 .. 4129  =  284 bp
ColE1-derived recombination site that helps to
maintain plasmids as monomers
SV40 poly(A) signal
1651 .. 1772  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1651 .. 1772  =  122 bp
SV40 polyadenylation signal
AmpR promoter
2019 .. 2123  =  105 bp
AmpR promoter
2019 .. 2123  =  105 bp
CMVd1 promoter
1 .. 100  =  100 bp
truncated human cytomegalovirus (CMV) immediate
early promoter that yields slightly reduced
expression relative to the full-length promoter
CMVd1 promoter
1 .. 100  =  100 bp
truncated human cytomegalovirus (CMV) immediate
early promoter that yields slightly reduced
expression relative to the full-length promoter
TEV site
588 .. 608  =  21 bp
7 amino acids  =  901.0 Da
Product: tobacco etch virus (TEV) protease
recognition and cleavage site
TEV protease recognition site
TEV site
588 .. 608  =  21 bp
7 amino acids  =  901.0 Da
Product: tobacco etch virus (TEV) protease
recognition and cleavage site
TEV protease recognition site
T7 promoter
132 .. 150  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
132 .. 150  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SP6 promoter
156 .. 174  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
156 .. 174  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter