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pHet-Mem1

Mammalian vector encoding FKBP heterodimerizer domains, for creating membrane-associated fusion proteins that can be dimerized with a drug. Also known as pC4M-F2E.

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pHet-Mem1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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PvuII (5614) NaeI (5412) NgoMIV (5410) DraIII (5309) PfoI (4894) XmnI (4460) AhdI (3849) BspQI - SapI (2840) MluI (1) SnaBI (298) EcoRI (656) AfeI (707) XbaI (710) AarI (712) TspMI - XmaI (831) SmaI (833) EagI (1075) SphI (1129) SacII (1213) KasI (1289) NarI (1290) SfoI (1291) PluTI (1293) AleI (1331) BmgBI (1342) SpeI (1364) BsiWI (1374) BamHI (1409) KflI (1438) HincII (1590) MfeI (1780) PspOMI (1955) ApaI (1959) MscI (2100) BstXI (2108) BglII (2129) PflMI (2335) AbsI - PaeR7I - PspXI - XhoI (2578) AvrII (2601) StuI (2606) SfiI (2655) pHet-Mem1 5726 bp
PvuII  (5614)
1 site
C A G C T G G T C G A C
NaeI  (5412)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (5410)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
DraIII  (5309)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PfoI  (4894)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
XmnI  (4460)
1 site
G A A N N N N T T C C T T N N N N A A G
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