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Plasmid Files

pSecTag2 B

Mammalian expression vector for the secretion of C-terminally 6xHis-tagged proteins. For other reading frames, use pSecTag2 A or pSecTag2 C.

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pSecTag2 B.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SgrDI (5161) SspI (5044) ScaI (4720) PvuI (4610) FspI (4462) AhdI (4240) PciI (3347) BspQI - SapI (3231) BstZ17I (2968) BsmI (2916) FseI (2608) BglII (12) MfeI (161) Bpu10I (180) NruI (208) MluI (228) SpeI (249) NdeI (484) SnaBI (590) NheI (895) BmtI (899) SfiI (977) BsiWI (995) HindIII (1001) Acc65I (1007) KpnI (1011) BamHI (1019) AleI (1028) EcoRI (1039) PstI (1048) EcoRV (1051) NotI (1066) PaeR7I - PspXI - XhoI (1072) EcoO109I - PspOMI (1080) ApaI (1084) PmeI (1155) BbsI (1366) StuI (2202) AvrII (2203) MscI (2335) SgrAI (2446) BmgBI (2466) pSecTag2 B 5163 bp
SgrDI  (5161)
1 site
C G T C G A C G G C A G C T G C
SspI  (5044)
1 site
A A T A T T T T A T A A
ScaI  (4720)
1 site
A G T A C T T C A T G A
PvuI  (4610)
1 site
C G A T C G G C T A G C
FspI  (4462)
1 site
T G C G C A A C G C G T
AhdI  (4240)
1 site
G A C