Resources
Plasmid Files

pSecTag2 B

Mammalian expression vector for the secretion of C-terminally 6xHis-tagged proteins. For other reading frames, use pSecTag2 A or pSecTag2 C.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pSecTag2 B Sequence and MappSecTag2 B.dna
Map and Sequence File   
Sequence Author:  Invitrogen (Life Technologies)
Download Free Trial Get SnapGene Viewer

 SgrDI (5161) SspI (5044) ScaI (4720) PvuI (4610) FspI (4462) AhdI (4240) PciI (3347) BspQI - SapI (3231) BstZ17I (2968) BsmI (2916) AanI (2855) FseI (2608) BglII (12) MfeI (161) Bpu10I (180) NruI (208) MluI (228) SpeI (249) NdeI (484) SnaBI (590) CMV promoter NheI (895) BmtI (899) SfiI (977) BsiWI (995) HindIII (1001) Acc65I (1007) KpnI (1011) BamHI (1019) AleI (1028) BstXI (1035) EcoRI (1039) PstI (1048) EcoRV (1051) BstXI (1061) NotI (1066) PaeR7I - PspXI - XhoI (1072) EcoO109I - PspOMI (1080) ApaI (1084) PmeI (1155) BbsI (1366) AanI (1805) StuI (2202) AvrII (2203) MscI (2335) SgrAI (2446) BmgBI (2466) pSecTag2 B 5163 bp
SgrDI  (5161)
1 site
C G T C G A C G G C A G C T G C
SspI  (5044)
1 site
A A T A T T T T A T A A
ScaI  (4720)
1 site
A G T A C T T C A T G A
PvuI  (4610)
1 site
C G A T C G G C T A G C
FspI  (4462)
1 site
T G C G C A A C G C G T
AhdI  (4240)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (3347)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (3231)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3231)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
BstZ17I  (2968)
1 site
G T A T A C C A T A T G
BsmI  (2916)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
AanI  (2855)
2 sites
T T A T A A A A T A T T
FseI  (2608)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
BglII  (12)
1 site
A G A T C T T C T A G A
MfeI  (161)
1 site
C A A T T G G T T A A C
Bpu10I  (180)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
NruI  (208)
1 site
T C G C G A A G C G C T
MluI  (228)
1 site
A C G C G T T G C G C A
SpeI  (249)
1 site
A C T A G T T G A T C A
NdeI  (484)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
SnaBI  (590)
1 site
T A C G T A A T G C A T
NheI  (895)
1 site
G C T A G C C G A T C G
BmtI  (899)
1 site
G C T A G C C G A T C G
SfiI  (977)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BsiWI  (995)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
HindIII  (1001)
1 site
A A G C T T T T C G A A
Acc65I  (1007)
1 site
G G T A C C C C A T G G
KpnI  (1011)
1 site
G G T A C C C C A T G G
BamHI  (1019)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
AleI  (1028)
1 site
C A C N N N N G T G G T G N N N N C A C
BstXI  (1035)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EcoRI  (1039)
1 site
G A A T T C C T T A A G
PstI  (1048)
1 site
C T G C A G G A C G T C
EcoRV  (1051)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
BstXI  (1061)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
NotI  (1066)
1 site
G C G G C C G C C G C C G G C G
PaeR7I  (1072)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1072)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1072)
1 site
C T C G A G G A G C T C
EcoO109I  (1080)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PspOMI  (1080)
1 site
G G G C C C C C C G G G
ApaI  (1084)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PmeI  (1155)
1 site
G T T T A A A C C A A A T T T G
BbsI  (1366)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
AanI  (1805)
2 sites
T T A T A A A A T A T T
StuI  (2202)
1 site
A G G C C T T C C G G A
AvrII  (2203)
1 site
C C T A G G G G A T C C
MscI  (2335)
1 site
T G G C C A A C C G G T
SgrAI  (2446)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
BmgBI  (2466)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
AmpR
4167 .. 5027  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   4167 .. 4958  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4167 .. 5027  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   4959 .. 5027  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4167 .. 5027  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
3408 .. 3996  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3408 .. 3996  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
1447 .. 1875  =  429 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
1447 .. 1875  =  429 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
BleoR
2332 .. 2706  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and
Zeocin™
BleoR
2332 .. 2706  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and
Zeocin™
SV40 promoter
1889 .. 2218  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
1889 .. 2218  =  330 bp
SV40 enhancer and early promoter
bGH poly(A) signal
1177 .. 1401  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
1177 .. 1401  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 poly(A) signal
2836 .. 2957  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2836 .. 2957  =  122 bp
SV40 polyadenylation signal
MCS
970 .. 1085  =  116 bp
multiple cloning site
MCS
970 .. 1085  =  116 bp
multiple cloning site
AmpR promoter
5028 .. 5132  =  105 bp
AmpR promoter
5028 .. 5132  =  105 bp
Igκ leader
905 .. 967  =  63 bp
21 amino acids  =  2.4 kDa
Product: leader sequence from mouse
immunoglobulin κ light chain
Igκ leader
905 .. 967  =  63 bp
21 amino acids  =  2.4 kDa
Product: leader sequence from mouse
immunoglobulin κ light chain
EM7 promoter
2266 .. 2313  =  48 bp
synthetic bacterial promoter
EM7 promoter
2266 .. 2313  =  48 bp
synthetic bacterial promoter
Myc
1086 .. 1115  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
Myc
1086 .. 1115  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
6xHis
1131 .. 1148  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
1131 .. 1148  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
SV40 ori
2069 .. 2204  =  136 bp
SV40 origin of replication
SV40 ori
2069 .. 2204  =  136 bp
SV40 origin of replication
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter