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Plasmid Files

pSecTag A

Mammalian expression vector for the secretion of C-terminally 6xHis-tagged proteins (replaced by pSecTag2 A). For other reading frames, use pSecTag B or pSecTag C.

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pSecTag A.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SgrDI (5164) SspI (5047) ScaI (4723) PvuI (4613) FspI (4465) AhdI (4243) PciI (3350) BspQI - SapI (3234) BstZ17I (2971) BsmI (2919) FseI (2611) BglII (12) MfeI (161) Bpu10I (180) NruI (208) MluI (228) NdeI (484) SnaBI (590) NheI (895) BmtI (899) SfiI (977) AscI (985) BsiWI (991) HindIII (997) Acc65I (1003) KpnI (1007) BamHI (1015) EcoRI (1038) PstI (1047) EcoRV (1050) NotI (1065) PaeR7I - PspXI - XhoI (1071) XbaI (1077) EcoO109I - PspOMI (1083) ApaI (1087) PmeI (1158) BbsI (1369) StuI (2205) AvrII (2206) MscI (2338) MauBI (2371) SgrAI (2449) BmgBI (2469) pSecTag A 5166 bp
SgrDI  (5164)
1 site
C G T C G A C G G C A G C T G C
SspI  (5047)
1 site
A A T A T T T T A T A A