Resources
Plasmid Files

pTCP

Mammalian cell vector with a puromycin resistance marker, for expressing a cDNA from the CMV promoter.

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pTCP Sequence and MappTCP.dna
Map and Sequence File   
Sequence Author:  transOMIC
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 BamHI (584) EcoRV (617) HindIII (646) NotI (702) XbaI (723) pTCP 4930 bp
BamHI  (584)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
EcoRV  (617)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
HindIII  (646)
1 site
A A G C T T T T C G A A
NotI  (702)
1 site
G C G G C C G C C G C C G G C G
XbaI  (723)
1 site
T C T A G A A G A T C T
AmpR
3684 .. 4544  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   3684 .. 4475  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
3684 .. 4544  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   4476 .. 4544  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
3684 .. 4544  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
PuroR
1598 .. 2197  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
1598 .. 2197  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
ori
2928 .. 3513  =  586 bp
high-copy-number colE1/pMB1/pBR322/pUC origin
of replication
ori
2928 .. 3513  =  586 bp
high-copy-number colE1/pMB1/pBR322/pUC origin
of replication
f1 ori
815 .. 1243  =  429 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
815 .. 1243  =  429 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
CMV enhancer
4915 .. 364  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
4915 .. 364  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
1257 .. 1586  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
1257 .. 1586  =  330 bp
SV40 enhancer and early promoter
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 poly(A) signal
2356 .. 2477  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2356 .. 2477  =  122 bp
SV40 polyadenylation signal
AmpR promoter
4545 .. 4649  =  105 bp
AmpR promoter
4545 .. 4649  =  105 bp
SV40 ori
1437 .. 1572  =  136 bp
SV40 origin of replication
SV40 ori
1437 .. 1572  =  136 bp
SV40 origin of replication
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