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pTRE-Cycle1

Bidirectional expression vector for cycling the level of a protein by alternating expression and rapid degradation.

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pTRE-Cycle1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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EcoRI (3109) AgeI (3103) NdeI (3098) SacII (3094) ApaI (3089) EcoO109I (3086) PspOMI (3085) BglII (3079) Bsu36I (3073) PstI (3070) XbaI (3060) AatII (2868) ZraI (2866) SspI (2750) EarI (2741) XmnI (2545) ScaI (2426) TatI (2424) PvuI (2316) FspI (2168) AseI (2118) NmeAIII (2094) BsaI (2007) AhdI (1946) PaeR7I - PspXI - XhoI (1) Acc65I (335) KpnI (339) NcoI - StyI (350) AarI - BfuAI - BspMI (351) BsmBI (377) BsgI (379) BbsI (639) BamHI (676) PvuII (690) MluI (694) NheI (700) BmtI (704) EagI - NotI (707) BspDI - ClaI (715) HindIII (720) SalI (726) AccI (727) EcoRV (734) PciI (1053) DrdI (1161) HaeII (1301) AlwNI (1469) pTRE-Cycle1 3188 bp
EcoRI  (3109)
1 site
G A A T T C C T T A A G
AgeI  (3103)
1 site
A C C G G T T G G C C A
NdeI  (3098)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SacII  (3094)
1 site