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Plasmid Files

pTRE-Cycle2

Vector for expressing mCherry while cycling the level of a second protein by alternating expression and rapid degradation.

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pTRE-Cycle2.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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EcoRI (3813) AgeI (3807) NdeI (3802) SacII (3798) ApaI (3793) EcoO109I (3790) PspOMI (3789) BglII (3783) MscI (3745) BsaAI (3545) PstI - SbfI (3424) PflMI (3339) BbvCI - Bpu10I (3234) SgrAI - XcmI (3090) BsrGI (3071) XbaI (3060) AatII (2868) ZraI (2866) SspI (2750) EarI (2741) XmnI (2545) ScaI (2426) PvuI (2316) FspI (2168) AseI (2118) BsaI (2007) PaeR7I - PspXI - XhoI (1) Acc65I (335) KpnI (339) AarI - BfuAI - BspMI (351) BsmBI (377) BamHI (676) MluI (694) NheI (700) BmtI (704) EagI - NotI (707) BspDI - ClaI (715) HindIII (720) SalI (726) AccI (727) EcoRV (734) PciI (1053) pTRE-Cycle2 3892 bp
EcoRI  (3813)
1 site
G A A T T C C T T A A G
AgeI  (3807)
1 site
A C C G G T T G G C C A
NdeI  (3802)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SacII  (3798)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
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ApaI  (3793)
1 site
G G G C C C C C C G G G