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pTRE-Tight-BI-AcGFP1

Vector for co-expressing AcGFP1 and another gene with the Tet-On® Advanced or Tet-Off® Advanced system.

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pTRE-Tight-BI-AcGFP1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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EcoRI (3454) PluTI (3440) SfoI (3438) NarI (3437) KasI (3436) MscI (3379) AleI (3272) BstEII (3265) Bpu10I (3257) Bsu36I (3181) PfoI * (3167) BssHII (3122) DraIII (3006) EcoNI (2827) StyI (2823) EcoO109I - PpuMI (2820) PflMI (2757) BtgI (2756) XbaI * (2728) AatII (2536) ZraI (2534) SspI (2418) EarI (2409) XmnI (2213) ScaI (2094) PvuI (1984) FspI (1836) AseI (1786) PaeR7I - PspXI - XhoI (1) Acc65I (335) KpnI - TspMI - XmaI (339) SmaI (341) BamHI (344) MluI (362) NheI (368) BmtI (372) EagI - NotI (375) BspDI - ClaI (383) HindIII (388) SalI (394) AccI (395) EcoRV (402) PciI (721) NspI (725) DrdI (829) BseYI (1025) PspFI (1029) AlwNI (1137) AhdI (1614) BmrI (1654) BsaI (1675) BglI (1734) pTRE-Tight-BI-AcGFP1 3533 bp
EcoRI  (3454)
1 site
G A A T T C C T T A A G
PluTI  (3440)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
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