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Plasmid Files

pTRE-Tight-BI

Vector for co-expressing two genes with the Tet-On® Advanced or Tet-Off® Advanced system.

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pTRE-Tight-BI.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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EcoRI (2777) AgeI (2771) NdeI (2766) SacII (2762) BtgI (2759) ApaI (2757) EcoO109I (2754) PspOMI (2753) BglII (2747) Bsu36I (2741) PstI (2738) XbaI (2728) AatII (2536) ZraI (2534) SspI (2418) EarI (2409) XmnI (2213) ScaI (2094) TatI (2092) PvuI (1984) FspI (1836) AseI (1786) NmeAIII (1762) BsaI (1675) BmrI (1654) AhdI (1614) PaeR7I - PspXI - XhoI (1) Acc65I (335) KpnI - TspMI - XmaI (339) SmaI (341) BamHI (344) PvuII (358) MluI (362) NheI (368) BmtI (372) EagI - NotI (375) BspDI - ClaI (383) HindIII (388) SalI (394) AccI (395) EcoRV (402) PciI (721) NspI (725) DrdI (829) HaeII (969) BseYI (1025) PspFI (1029) AlwNI (1137) pTRE-Tight-BI 2856 bp
EcoRI  (2777)
1 site
G A A T T C C T T A A G
AgeI  (2771)
1 site
A C C G G T T G G C C A
NdeI  (2766)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SacII  (2762)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
BtgI  (2759)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
ApaI  (2757)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
EcoO109I  (2754)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PspOMI  (2753)
1 site
G G G C C C C C C G G G
BglII  (2747)
1 site
A G A T C T T C T A G A
Bsu36I  (2741)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PstI  (2738)
1 site
C T G C A G G A C G T C
XbaI  (2728)
1 site
T C T A G A A G A T C T
AatII  (2536)
1 site
G A C G T C C T G C A G
ZraI  (2534)
1 site
G A C G T C C T G C A G
SspI  (2418)
1 site
A A T A T T T T A T A A
EarI  (2409)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI recognition sequence.
Sticky ends from different EarI sites may not be compatible.
XmnI  (2213)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (2094)
1 site
A G T A C T T C A T G A
TatI  (2092)
1 site
W G T A C W W C A T G W
PvuI  (1984)
1 site
C G A T C G G C T A G C
FspI  (1836)
1 site
T G C G C A A C G C G T
AseI  (1786)
1 site
A T T A A T T A A T T A
NmeAIII  (1762)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaI  (1675)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BmrI  (1654)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
AhdI  (1614)
1 site
G A C N N