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Plasmid Files

pTetOne™

All-in-one vector for strong inducible expression of genes in mammalian cells using the Tet-On® system.

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pTetOne.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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NotI (4195) MluI (4188) AgeI (4176) BamHI (4170) BglII (4164) PstI (4162) AatII (3954) ZraI (3952) SspI (3836) XmnI (3631) PvuI (3402) AseI (3204) BglI (3152) AlwNI (2555) DrdI (2247) PciI (2139) EcoRI (4202) HindIII (114) BsgI (375) AarI (397) BstXI (548) StyI (566) PfoI (615) BsmBI - SpeI (801) MscI (867) BlpI (881) AscI (890) BspEI (995) StuI (1144) BsaAI - PmlI (1205) BseRI (1332) NruI (1354) TspMI - XmaI (1812) SmaI (1814) pTetOne™ 4207 bp
NotI  (4195)
1 site
G C G G C C G C C G C C G G C G
MluI  (4188)
1 site
A C G C G T T G C G C A
AgeI  (4176)
1 site
A C C G G T T G G C C A
BamHI  (4170)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BglII  (4164)
1 site
A G A T C T T C T A G A
PstI  (4162)
1 site
C T G C A G G A C G T C
AatII  (3954)
1 site
G A C G T C C T G C A G
ZraI  (3952)
1 site
G A C G T C C T G C A G
SspI  (3836)
1 site
A A T A T T T T A T A A
XmnI  (3631)
1 site
G A A N N N N T T