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Plasmid Files

pTracer™-CMV

Mammalian vector with a CMV promoter, for co-expression with GFP fused to a Zeocin™ resistance marker. See also pTracer™‑CMV2.

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pTracer-CMV.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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PmeI (904) AflII (908) Acc65I (917) KpnI (921) BstXI (948) EcoRI (952) PstI (961) EcoRV (964) BstXI (974) NotI (979) XbaI (991) EcoO109I - PspOMI (997) ApaI (1001) PmeI (1006) pTracer™-CMV 5717 bp
PmeI  (904)
2 sites
G T T T A A A C C A A A T T T G
AflII  (908)
1 site
C T T A A G G A A T T C
Acc65I  (917)
1 site
G G T A C C C C A T G G
KpnI  (921)
1 site
G G T A C C C C A T G G
BstXI  (948)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EcoRI  (952)
1 site
G A A T T C C T T A A G
PstI  (961)
1 site
C T G C A G G A C G T C
EcoRV  (964)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BstXI  (974)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
NotI  (979)
1 site
G C G G C C G C C G C C G G C G
XbaI  (991)
1 site
T C T A G A A G A T C T
EcoO109I  (997)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PspOMI  (997)
1 site
G G G C C C C C C G G G
ApaI  (1001)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PmeI  (1006)
2 sites
G T T T A A A C C A A A T T T G
AmpR
4721 .. 5581  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   4721 .. 5512  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4721 .. 5581  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   5513 .. 5581  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4721 .. 5581  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
Cycle 3 GFP
2184 .. 2882  =  699 bp
233 amino acids  =  26.1 kDa
Product: Cycle 3 GFP (Crameri et al., 1996)
mammalian codon-optimized
Cycle 3 GFP
2184 .. 2882  =  699 bp
233 amino acids  =  26.1 kDa
Product: Cycle 3 GFP (Crameri et al., 1996)
mammalian codon-optimized
ori
3962 .. 4550  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3962 .. 4550  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1298 .. 1726  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1298 .. 1726  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
BleoR
2883 .. 3260  =  378 bp
125 amino acids  =  13.9 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
BleoR
2883 .. 3260  =  378 bp
125 amino acids  =  13.9 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
SV40 promoter
1740 .. 2070  =  331 bp
SV40 enhancer and early promoter
SV40 promoter
1740 .. 2070  =  331 bp
SV40 enhancer and early promoter
bGH poly(A) signal
1028 .. 1252  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
1028 .. 1252  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
3390 .. 3511  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3390 .. 3511  =  122 bp
SV40 polyadenylation signal
MCS
901 .. 1010  =  110 bp
multiple cloning site
MCS
901 .. 1010  =  110 bp
multiple cloning site
AmpR promoter
5582 .. 5686  =  105 bp
AmpR promoter
5582 .. 5686  =  105 bp
EM7 promoter
2118 .. 2165  =  48 bp
synthetic bacterial promoter
EM7 promoter
2118 .. 2165  =  48 bp
synthetic bacterial promoter
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
1921 .. 2056  =  136 bp
SV40 origin of replication
SV40 ori
1921 .. 2056  =  136 bp
SV40 origin of replication
ORF:  1249 .. 1515  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  2184 .. 3260  =  1077 bp
ORF:  358 amino acids  =  40.0 kDa
ORF:  4851 .. 5117  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2783 .. 3166  =  384 bp
ORF:  127 amino acids  =  13.7 kDa
ORF:  4721 .. 5581  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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