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Plasmid Files

pTracer™-CMV/Bsd

Mammalian vector with a CMV promoter, for co-expression with GFP fused to a blasticidin resistance marker.

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pTracer-CMV Bsd.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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NheI (895) BmtI (899) PmeI (904) BstXI (948) EcoRI (952) EcoRV (964) BstXI (974) NotI (979) XbaI (991) PmeI (1006) BclI * (1015) pTracer™-CMV/Bsd 6048 bp
NheI  (895)
1 site
G C T A G C C G A T C G
BmtI  (899)
1 site
G C T A G C C G A T C G
PmeI  (904)
2 sites
G T T T A A A C C A A A T T T G
BstXI  (948)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EcoRI  (952)
1 site
G A A T T C C T T A A G
EcoRV  (964)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BstXI  (974)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
NotI  (979)
1 site
G C G G C C G C C G C C G G C G
XbaI  (991)
1 site
T C T A G A A G A T C T
PmeI  (1006)
2 sites
G T T T A A A C C A A A T T T G
BclI  (1015)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
EF-1α promoter
1307 .. 2485  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
EF-1α promoter
1307 .. 2485  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
AmpR
5052 .. 5912  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   5052 .. 5843  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5052 .. 5912  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   5844 .. 5912  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5052 .. 5912  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
Cycle 3 GFP
2576 .. 3274  =  699 bp
233 amino acids  =  26.1 kDa
Product: Cycle 3 GFP (Crameri et al., 1996)
Cycle 3 GFP
2576 .. 3274  =  699 bp
233 amino acids  =  26.1 kDa
Product: Cycle 3 GFP (Crameri et al., 1996)
ori
4293 .. 4881  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4293 .. 4881  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
BSD
3284 .. 3679  =  396 bp
131 amino acids  =  13.5 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
BSD
3284 .. 3679  =  396 bp
131 amino acids  =  13.5 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
3721 .. 3842  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3721 .. 3842  =  122 bp
SV40 polyadenylation signal
bGH poly(A) signal
1028 .. 1139  =  112 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
1028 .. 1139  =  112 bp
bovine growth hormone polyadenylation signal
AmpR promoter
5913 .. 6017  =  105 bp
AmpR promoter
5913 .. 6017  =  105 bp
EM7 promoter
2510 .. 2557  =  48 bp
synthetic bacterial promoter
EM7 promoter
2510 .. 2557  =  48 bp
synthetic bacterial promoter
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
EF-1α intron A
1538 .. 2476  =  939 bp
intron upstream of the start codon of human EF-1α
EF-1α intron A
1538 .. 2476  =  939 bp
intron upstream of the start codon of human EF-1α
ORF:  1942 .. 2175  =  234 bp
ORF:  77 amino acids  =  7.7 kDa
ORF:  5182 .. 5448  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2576 .. 3679  =  1104 bp
ORF:  367 amino acids  =  40.0 kDa
ORF:  1863 .. 2300  =  438 bp
ORF:  145 amino acids  =  15.9 kDa
ORF:  3270 .. 3500  =  231 bp
ORF:  76 amino acids  =  8.3 kDa
ORF:  1605 .. 2225  =  621 bp
ORF:  206 amino acids  =  23.0 kDa
ORF:  5052 .. 5912  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3161 .. 3583  =  423 bp
ORF:  140 amino acids  =  15.4 kDa
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