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Plasmid Files

pTracer™-EF/V5-His A

Mammalian vector with an EF-1α promoter, for co-expression with GFP fused to a Zeocin™ resistance marker. See also pTracer™‑EF/V5-His B and pTracer™‑EF/V5-His C.

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pTracer-EF V5-His A.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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Acc65I (1715) KpnI (1719) SpeI (1733) BstXI (1746) EcoRI (1750) EcoRV (1762) BstXI (1772) NotI (1777) XbaI (1789) PmeI (1882) pTracer™-EF/V5-His A 5948 bp
Acc65I  (1715)
1 site
G G T A C C C C A T G G
KpnI  (1719)
1 site
G G T A C C C C A T G G
SpeI  (1733)
1 site
A C T A G T T G A T C A
BstXI  (1746)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EcoRI  (1750)
1 site
G A A T T C C T T A A G
EcoRV  (1762)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BstXI  (1772)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
NotI  (1777)
1 site
G C G G C C G C C G C C G G C G
XbaI  (1789)
1 site
T C T A G A A G A T C T
PmeI  (1882)
1 site
G T T T A A A C C A A A T T T G
V5 tag
1807 .. 1848  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
1807 .. 1848  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
6xHis
1858 .. 1875  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
1858 .. 1875  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
EF-1α promoter
475 .. 1653  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
EF-1α promoter
475 .. 1653  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
AmpR
5074 .. 5934  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   5074 .. 5865  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5074 .. 5934  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   5866 .. 5934  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5074 .. 5934  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
Cycle 3 GFP
2865 .. 3563  =  699 bp
233 amino acids  =  26.1 kDa
Product: Cycle 3 GFP (Crameri et al., 1996)
mammalian codon-optimized
Cycle 3 GFP
2865 .. 3563  =  699 bp
233 amino acids  =  26.1 kDa
Product: Cycle 3 GFP (Crameri et al., 1996)
mammalian codon-optimized
ori
4315 .. 4903  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4315 .. 4903  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
BleoR
3564 .. 3941  =  378 bp
125 amino acids  =  13.9 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
BleoR
3564 .. 3941  =  378 bp
125 amino acids  =  13.9 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
CMV enhancer
2159 .. 2462  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2159 .. 2462  =  304 bp
human cytomegalovirus immediate early enhancer
bGH poly(A) signal
1904 .. 2128  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
1904 .. 2128  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
2463 .. 2666  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
2463 .. 2666  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
3955 .. 4089  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3955 .. 4089  =  135 bp
SV40 polyadenylation signal
AmpR promoter
5935 .. 91  =  105 bp
AmpR promoter
5935 .. 91  =  105 bp
EM7 promoter
2784 .. 2831  =  48 bp
synthetic bacterial promoter
EM7 promoter
2784 .. 2831  =  48 bp
synthetic bacterial promoter
T7 promoter
1670 .. 1688  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1670 .. 1688  =  19 bp
promoter for bacteriophage T7 RNA polymerase
EF-1α intron A
706 .. 1644  =  939 bp
intron upstream of the start codon of human EF-1α
EF-1α intron A
706 .. 1644  =  939 bp
intron upstream of the start codon of human EF-1α
MCS
1715 .. 1886  =  172 bp
multiple cloning site
MCS
1715 .. 1886  =  172 bp
multiple cloning site
ORF:  1031 .. 1468  =  438 bp
ORF:  145 amino acids  =  15.9 kDa
ORF:  2102 .. 2356  =  255 bp
ORF:  84 amino acids  =  9.5 kDa
ORF:  5204 .. 5470  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1110 .. 1343  =  234 bp
ORF:  77 amino acids  =  7.7 kDa
ORF:  2850 .. 3941  =  1092 bp
ORF:  363 amino acids  =  40.5 kDa
ORF:  773 .. 1393  =  621 bp
ORF:  206 amino acids  =  23.0 kDa
ORF:  3464 .. 3847  =  384 bp
ORF:  127 amino acids  =  13.7 kDa
ORF:  4064 .. 4345  =  282 bp
ORF:  93 amino acids  =  10.2 kDa
ORF:  2545 .. 2805  =  261 bp
ORF:  86 amino acids  =  9.3 kDa
ORF:  5074 .. 5934  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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