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Plasmid Files

pTracer™-SV40

Mammalian vector with an SV40 promoter, for co-expression with GFP fused to a Zeocin™ resistance marker.

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pTracer-SV40.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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PmeI (442) AflII (446) Acc65I (455) KpnI (459) SpeI (473) BstXI (486) EcoRI (490) PstI (499) EcoRV (502) BstXI (512) NotI (517) PspOMI (535) ApaI (539) PmeI (544) pTracer™-SV40 4217 bp
PmeI  (442)
2 sites
G T T T A A A C C A A A T T T G
AflII  (446)
1 site
C T T A A G G A A T T C
Acc65I  (455)
1 site
G G T A C C C C A T G G
KpnI  (459)
1 site
G G T A C C C C A T G G
SpeI  (473)
1 site
A C T A G T T G A T C A
BstXI  (486)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EcoRI  (490)
1 site
G A A T T C C T T A A G
PstI  (499)
1 site
C T G C A G G A C G T C
EcoRV  (502)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BstXI  (512)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
NotI  (517)
1 site
G C G G C C G C C G C C G G C G
PspOMI  (535)
1 site
G G G C C C C C C G G G
ApaI  (539)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PmeI  (544)
2 sites
G T T T A A A C C A A A T T T G
Cycle 3 GFP
2998 .. 3696  =  699 bp
233 amino acids  =  26.1 kDa
Product: Cycle 3 GFP (Crameri et al., 1996)
mammalian codon-optimized
Cycle 3 GFP
2998 .. 3696  =  699 bp
233 amino acids  =  26.1 kDa
Product: Cycle 3 GFP (Crameri et al., 1996)
mammalian codon-optimized
ori
873 .. 1461  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
873 .. 1461  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1816 .. 2271  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1816 .. 2271  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
BleoR
3697 .. 4074  =  378 bp
125 amino acids  =  13.9 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
BleoR
3697 .. 4074  =  378 bp
125 amino acids  =  13.9 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
SV40 promoter
22 .. 351  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
22 .. 351  =  330 bp
SV40 enhancer and early promoter
CMV enhancer
2307 .. 2610  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2307 .. 2610  =  304 bp
human cytomegalovirus immediate early enhancer
bGH poly(A) signal
566 .. 790  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
566 .. 790  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
2611 .. 2814  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
2611 .. 2814  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
4088 .. 4209  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
4088 .. 4209  =  122 bp
SV40 polyadenylation signal
MCS
439 .. 548  =  110 bp
multiple cloning site
MCS
439 .. 548  =  110 bp
multiple cloning site
EM7 promoter
2932 .. 2979  =  48 bp
synthetic bacterial promoter
EM7 promoter
2932 .. 2979  =  48 bp
synthetic bacterial promoter
T7 promoter
401 .. 419  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
401 .. 419  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
202 .. 337  =  136 bp
SV40 origin of replication
SV40 ori
202 .. 337  =  136 bp
SV40 origin of replication
ORF:  2998 .. 4074  =  1077 bp
ORF:  358 amino acids  =  40.0 kDa
ORF:  764 .. 1198  =  435 bp
ORF:  144 amino acids  =  16.3 kDa
ORF:  1788 .. 2033  =  246 bp
ORF:  81 amino acids  =  8.6 kDa
ORF:  3597 .. 3980  =  384 bp
ORF:  127 amino acids  =  13.7 kDa
ORF:  2693 .. 2953  =  261 bp
ORF:  86 amino acids  =  9.3 kDa
ORF:  556 .. 903  =  348 bp
ORF:  115 amino acids  =  12.7 kDa
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