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Plasmid Files

pZFHD1-2

Mammalian vector encoding a promoter that can be activated by drug-induced heterodimerization of ZFHD1 and the p65 activation domain.

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pZFHD1-2 Sequence and MappZFHD1-2.dna
Map and Sequence File   
Sequence Author:  Clontech
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 AfeI (14) BsgI (3930) AarI (3902) ScaI (3837) TatI (3835) ApaLI (3459) BsaI (2844) PfoI (2630) RsrII (2371) BsrDI (2088) PflFI - Tth111I (1973) BglII (27) Eco53kI (36) SacI (38) Acc65I (63) KpnI (67) SacII (70) MluI (76) NheI (82) BmtI (86) AseI (311) XmnI (312) AgeI - SgrAI (462) PspOMI (468) ApaI (472) TspMI - XmaI (474) SmaI (476) BamHI (480) SpeI (486) BclI * (492) NotI (499) MfeI (605) HpaI (618) Bts α I (694) AflII (737) DraIII (971) SexAI * (1444) BglI - SfiI (1630) BseRI (1673) StuI (1676) KasI (1854) NarI (1855) SfoI (1856) PluTI (1858) MscI (1937) FspI (1957) pZFHD1-2 3991 bp
AfeI  (14)
1 site
A G C G C T T C G C G A
BsgI  (3930)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AarI  (3902)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
ScaI  (3837)
1 site
A G T A C T T C A T G A
TatI  (3835)
1 site
W G T A C W W C A T G W
ApaLI  (3459)
1 site
G T G C A C C A C G T G
BsaI  (2844)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (2630)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (2371)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2088)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (1973)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1973)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BglII  (27)
1 site
A G A T C T T C T A G A
Eco53kI  (36)
1 site
G A G C T C C T C G A G
SacI  (38)
1 site
G A G C T C C T C G A G
Acc65I  (63)
1 site
G G T A C C C C A T G G
KpnI  (67)
1 site
G G T A C C C C A T G G
SacII  (70)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
MluI  (76)
1 site
A C G C G T T G C G C A
NheI  (82)
1 site
G C T A G C C G A T C G
BmtI  (86)
1 site
G C T A G C C G A T C G
AseI  (311)
1 site
A T T A A T T A A T T A
XmnI  (312)
1 site
G A A N N N N T T C C T T N N N N A A G
AgeI  (462)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
SgrAI  (462)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
PspOMI  (468)
1 site
G G G C C C C C C G G G
ApaI  (472)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
TspMI  (474)
1 site
C C C G G G G G G C C C
XmaI  (474)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (476)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (480)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
SpeI  (486)
1 site
A C T A G T T G A T C A
BclI  (492)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
NotI  (499)
1 site
G C G G C C G C C G C C G G C G
MfeI  (605)
1 site
C A A T T G G T T A A C
HpaI  (618)
1 site
G T T A A C C A A T T G
BtsαI  (694)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
AflII  (737)
1 site
C T T A A G G A A T T C

The sticky ends produced by AflII are hard to ligate.
DraIII  (971)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SexAI  (1444)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BglI  (1630)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SfiI  (1630)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BseRI  (1673)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
StuI  (1676)
1 site
A G G C C T T C C G G A
KasI  (1854)
1 site
G G C G C C C C G C G G
NarI  (1855)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
SfoI  (1856)
1 site
G G C G C C C C G C G G
PluTI  (1858)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
MscI  (1937)
1 site
T G G C C A A C C G G T
FspI  (1957)
1 site
T G C G C A A C G C G T
NeoR/KanR
1727 .. 2521  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
NeoR/KanR
1727 .. 2521  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
ori
3129 .. 3717  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3129 .. 3717  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
747 .. 1202  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
747 .. 1202  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
SV40 promoter
1335 .. 1692  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
1335 .. 1692  =  358 bp
SV40 enhancer and early promoter
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 1:  
   88 .. 99  =  12 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 2:  
   100 .. 105  =  6 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 3:  
   106 .. 117  =  12 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 4:  
   118 .. 123  =  6 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 5:  
   124 .. 135  =  12 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 6:  
   136 .. 141  =  6 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 7:  
   142 .. 153  =  12 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 8:  
   154 .. 159  =  6 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 9:  
   160 .. 171  =  12 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 10:  
   172 .. 177  =  6 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 11:  
   178 .. 189  =  12 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 12:  
   190 .. 195  =  6 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 13:  
   196 .. 207  =  12 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 14:  
   208 .. 213  =  6 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 15:  
   214 .. 225  =  12 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 16:  
   226 .. 231  =  6 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 17:  
   232 .. 243  =  12 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 18:  
   244 .. 249  =  6 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 19:  
   250 .. 261  =  12 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 20:  
   262 .. 267  =  6 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 21:  
   268 .. 279  =  12 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 22:  
   280 .. 285  =  6 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
   Segment 23:  
   286 .. 297  =  12 bp
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
ZFHD1 binding sites
88 .. 297  =  210 bp
23 segments
12 binding sites for the composite human
DNA-binding domain ZFHD1 (Pomerantz et al.,
1995)
SV40 poly(A) signal
619 .. 740  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
619 .. 740  =  122 bp
SV40 polyadenylation signal
minimal IL-2 promoter
320 .. 433  =  114 bp
minimal promoter from the human interleukin-2
gene
minimal IL-2 promoter
320 .. 433  =  114 bp
minimal promoter from the human interleukin-2
gene
AmpR promoter
1229 .. 1333  =  105 bp
AmpR promoter
1229 .. 1333  =  105 bp
pause site
3841 .. 3932  =  92 bp
RNA polymerase II transcriptional pause signal from
the human α2 globin gene
pause site
3841 .. 3932  =  92 bp
RNA polymerase II transcriptional pause signal from
the human α2 globin gene
poly(A) signal
3779 .. 3827  =  49 bp
synthetic polyadenylation signal
poly(A) signal
3779 .. 3827  =  49 bp
synthetic polyadenylation signal
HSV TK poly(A) signal
2753 .. 2800  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
2753 .. 2800  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
MCS
462 .. 505  =  44 bp
multiple cloning site
MCS
462 .. 505  =  44 bp
multiple cloning site
SV40 ori
1543 .. 1678  =  136 bp
SV40 origin of replication
SV40 ori
1543 .. 1678  =  136 bp
SV40 origin of replication
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