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Plasmid Files

pcDNA™3.1/CT-GFP

Vector for expressing C-terminally GFP-tagged proteins in mammalian cells.

To see this sequence with restriction sites, features, and translations, please download
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pcDNA3.1 CT-GFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SgrDI (6139) SspI (6022) ScaI (5698) PvuI (5588) AhdI (5218) PciI (4325) BsmI (3894) PfoI (3749) RsrII (3488) BssHII (3369) PflFI - Tth111I (3090) PluTI (2975) BglII (12) Bpu10I (180) SnaBI (590) Acc65I (908) KpnI (912) EcoRI (943) EcoRV (955) NotI (970) XbaI (982) NheI (991) BmtI (995) BspEI * (1212) BsrGI (1266) BstBI (1612) BbsI (1924) DraIII (2238) SexAI * (2529) BseRI (2758) StuI (2761) AvrII (2762) TspMI - XmaI (2783) SmaI (2785) BsaBI * (2831) KasI (2971) NarI (2972) SfoI (2973) pcDNA™3.1/CT-GFP 6141 bp
SgrDI  (6139)
1 site
C G T C G A C G G C A G C T G C
SspI  (6022)
1 site
A A T A T T T T A T A A
ScaI  (5698)
1 site
A G T A C T T C A T G A
PvuI  (5588)
1 site
C G A T C G G C T A G C
AhdI  (5218)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (4325)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BsmI  (3894)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
PfoI  (3749)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3488)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BssHII  (3369)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
PflFI  (3090)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3090)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PluTI  (2975)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BglII  (12)
1 site
A G A T C T T C T A G A
Bpu10I  (180)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
SnaBI  (590)
1 site
T A C G T A A T G C A T
Acc65I  (908)
1 site